custom-made
Slc6a14
Spezies: Maus
Wirt: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Slc6a14
(Solute Carrier Family 6 (Amino Acid Transporter), Member 14 (Slc6a14))
Protein-Typ
Recombinant
Proteineigenschaft
AA 1-638
Spezies
Maus
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses Slc6a14 Protein ist gelabelt mit His tag.
Verwendungszweck
Custom-made recombinant Slc6a14 Protein expressed in mammalian cells.
Sequenz
MDRLKCPNFF KCRQKEKVTA SSENFHVGEN DENQERGNWS KKSDYLLSMV GYAVGLGNVW RFPYLTYTNG GGAFLIPYAI MLALAGLPLF FLECSLGQFA SLGPVSVWRI LPLFQGVGIT MVLISVFVAI YYNVIIAYSL YYLFASFQSV LPWANCSSWA DENCSRTPIV TGCNVSIGAG EMFMNISWVN TNNLTCLNGS EVFRPGQLPS EQYWDKVTLQ RSSGMDETGV VVWYLALCLL LAWLIVGAAL FKGIKSSGKV VYFTALFPYV VLLILLIRGA TLEGASKGIS YYIGAQSNFT KLREAEVWKD AATQIFYSLS VAWGGLVALS SYNKFNNNCY SDAIIVCLTN CLTSVFAGFA IFSILGHMAH ISGKEVSQVV KSGFDLAFIA YPEALAQLPA GPFWSILFFF MLLTLGLDSQ FASIETITTT FQDLFPKAMK RMRVPITLGC CLILFLLGLL CVTQAGIYWV HLIDHFCAGW GILIAAILEI AGIIWIYGGN RFIEDIEMMI GAKRWIFWLW WRACWFVITP ILLSAILVWS LVKFHRPDYA DIPYPDWGVA LGWCMIIFCI IWIPIMAIIK IVQAEGNILQ RIISCCRPAS NWGPYLEKHR GERYRDMAEP AKETDHEIPT ISGSTKPE Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
Spezifität
If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
Produktmerkmale
Key Benefits:
Made to order protein - from design to production - by highly experienced protein experts.
Protein expressed in mammalian cells and purified in one-step affinity chromatography
The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Reinheit
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
custom-made
Slc6a14
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
custom-made
Slc6a14
Spezies: Human
Wirt: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, SDS, WB
custom-made
Slc6a14
Spezies: Maus
Wirt: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, SDS, WB
Applikationshinweise
We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
The buffer composition is at the discretion of the manufacturer.
Handhabung
Avoid repeated freeze-thaw cycles.
Lagerung
-80 °C
Informationen zur Lagerung
Store at -80°C.
Haltbarkeit
12 months
Target
Slc6a14
(Solute Carrier Family 6 (Amino Acid Transporter), Member 14 (Slc6a14))
Sodium- and chloride-dependent neutral and basic amino acid transporter B(0+) (Amino acid transporter ATB0+) (Colonic system B0+ amino acid transporter CATB0+) (Solute carrier family 6 member 14),FUNCTION: Amino acid transporter that plays an important role in the absorption of amino acids in the intestinal tract. Mediates the uptake of a broad range of neutral and cationic amino acids (with the exception of proline) in a Na(+)/Cl(-)-dependent manner (PubMed:11306607, PubMed:11447016). Transports non-alpha-amino acids such as beta-alanine with low affinity, and has a higher affinity for dipolar and cationic amino acids such as leucine and lysine (By similarity). Can also transport carnitine, butyrylcarnitine and propionylcarnitine coupled to the transmembrane gradients of Na(+) and Cl(-) (PubMed:11306651). {ECO:0000250|UniProtKB:Q9UN76, ECO:0000269|PubMed:11306607, ECO:0000269|PubMed:11306651, ECO:0000269|PubMed:11447016}.