MFSD8 Protein (AA 1-519) (His tag)

Produktnummer ABIN7558605

Produktdetails

Target: MFSD8 (Major Facilitator Superfamily Domain Containing 8 (MFSD8))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-519

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses MFSD8 Protein ist gelabelt mit His tag.

Verwendungszweck: Made-to-order recombinant Mfsd8 Protein expressed in mammalian cells.

Sequenz: MANLGSEAER EPLLGPGSPG SREWSEIETQ EHYKSRWKSV RILYLTMFLS SVGFSIVIMS IWPYLQKIDQ TADASFLGWV IASYSLGQMV ASPLFGLWSN YRPRKEPLIV SISISVAANC LYAYVHVPAA HNKYYMLIAR GLVGFGAGNV AVVRSYIAGA TSLQERTNAM ANTSTCQALG FILGPVFQTC FALIGEKGVT WDIIKLQVNM YTAPVLLAAF LGILNIILIL FILREHRVDD LGRQCKSVNF QEENTDEPQI PEGSIDQVAV VATNIVFFVV LFIFAVYETI LTPLTLDMYA WTQEQAVLYD GILLVAFGVE AVLVFMGVKL LSKKIGERAI LLGGFVVVWV GFFILLPWGN QFPKIQWEDL HNSSTPNTTF GEIIIGLWNS SREDHSEQPT GCPIEQTWCL YTPVIHLAQF LTAAVLIGTG YPACSVMSYT LYSKVLGPKP QGIYMGWLTT SGSAARILGP VFISHVYTYL GPRWAFSLVC GIVVLTILLI GAVYKRLVAF SVRYMRIQE Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Spezifität: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Produktmerkmale: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Reinheit: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Güteklasse: custom-made

Anwendungsinformationen

Applikationshinweise: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: MFSD8 (Major Facilitator Superfamily Domain Containing 8 (MFSD8))

Andere Bezeichnung: Mfsd8 (MFSD8 Produkte)

Hintergrund: Major facilitator superfamily domain-containing protein 8,FUNCTION: Outward-rectifying chloride channel involved in endolysosomal chloride homeostasis, membrane fusion and function. Conducts chloride currents up to hundreds of picoamperes. Regulates lysosomal calcium content by reducing the lysosomal membrane potential, thereby activating TRPML1 channel and further release of lysosomal calcium ions. Regulates the  pH in endolysosomal compartments and may contribute to progressive acidification from endosome to lysosome. Permeable to other halides such as iodide and fluoride ions. {ECO:0000250|UniProtKB:Q8NHS3, ECO:0000269|PubMed:34910516}.

Molekulargewicht: 57.6 kDa

UniProt: Q8BH31

AlphaFold: Q8BH31