Nuclease Protein

Produktnummer ABIN7520371

Dieses Recombinant Nuclease-Protein wird in Yeast (Pichia pastoris) produziert.

Kurzübersicht für Nuclease Protein (ABIN7520371)

Target: Nuclease

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Serratia marcescens

Quelle: Yeast (Pichia pastoris)

Reinheit: > 95 % by SDS-PAGE.

Produktdetails

Verwendungszweck: Active Recombinant Serratia marcescens Nuclease

Sequenz: DTLESIDNCA VGCPTGGSSN VSIVRHAYTL NNNSTTKFAN WVAYHITKDT PASGKTRNWK TDPALNPADT LAPADYTGAN AALKVDRGHQ APLASLAGVS DWESLNYLSN ITPQKSDLNQ GAWARLEDQE RKLIDRADIS SVYTVTGPLY ERDMGKLPGT QKAHTIPSAY WKVIFINNSP AVNHYAAFLF DQNTPKGADF CQFRVTVDEI EKRTGLIIWA GLPDDVQASL KSKPGVLPEL MGCKN

Spezifität: Asp22-Asn266

Endotoxin-Niveau: <0.1EU/μg

Biological Activity Comment: 1.One unit (U) is defined as the amount of enzyme required to change the absorption value of A260 by 1.0 (equivalent to complete digestion of 37 μg of salmon essence DNA into oligonucleotides) in 30 min at 37°C, pH 8.0 reaction conditions. The specific activity of Serratia marcescens nuclease is > 1000 U/μL.|2.Recombinant Serratia marcescens Nuclease (0.1U) can effectively degrade 5 μg plasmid DNA at 37°C for 30 min. The reaction buffer is: 10 mM MgCl2, 0.1 mg/mL BSA, 50 mM Tris-HCl, pH8.5.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Solution in 50 % glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl₂, and 20 mM NaCl.

Lagerung: -20 °C

Informationen zur Lagerung: This product is stable at -15°C ~ -25°C for up to 2 year from the date of receipt.|For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.

Antigendetails

Target: Nuclease

Hintergrund:

Description: Catalyzes the hydrolysis of both DNA and RNA, double- or single-stranded, at the 3'position of the phosphodiester bond to produce 5'-phosphorylated mono-, di-, tri- and tetranucleotides. DNA is a slightly better substrate than RNA.

Name: Endonuclease, Nuclease, nucA, nuc

Gen-ID: 66715254

UniProt: P13717