Periostin Protein (POSTN) (AA 22-836) (His tag)

Produktnummer ABIN7275381

Produktdetails

Target: Periostin (POSTN)

Protein-Typ: Recombinant

Proteineigenschaft: AA 22-836

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Periostin Protein ist gelabelt mit His tag.

Verwendungszweck: Human Periostin/OSF-2 Protein

Sequenz: Asn22-Gln836

Produktmerkmale: Recombinant Human Periostin/OSF-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asn22-Gln836.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Periostin, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 75.7ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Periostin (POSTN)

Andere Bezeichnung: Periostin (POSTN Produkte)

Hintergrund: Periostin is a matricellular protein that is expressed in several tissues during embryonic development, however, its expression in adults is mostly restricted to collagen-rich connective tissues. Periostin is expressed only briefly during kidney development, but it is not normally detected in the adult kidney. Recent evidence has revealed that periostin is aberrantly expressed in several forms of chronic kidney disease (CKD), and that its expression correlates with the degree of interstitial fibrosis and the decline in renal function.

Molekulargewicht: 92.1 kDa. Due to glycosylation, the protein migrates to 93-100 kDa based on Tris-Bis PAGE result.

UniProt: Q15063