Integrin alpha V beta 6 (ITGAV & ITGB6) Heterodimer (AA 31-992) protein (His-Avi Tag)
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Target
- Integrin alpha V beta 6 (ITGAV & ITGB6) Heterodimer
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 31-992
- Spezies
- Human
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- His-Avi Tag
- Verwendungszweck
- Human Integrin alpha V beta 6 (ITGAV & ITGB6) Heterodimer Protein
- Sequenz
- Phe31-Val992 (ITGAV) acidic tail & Gly22-Asn707 (ITGB6) basic tail
- Spezifität
- Uni-Prot: P06756-1 (ITGAV), P18564-1 (ITGB6)
- Produktmerkmale
- Recombinant Human Integrin alpha V beta 6 (ITGAV & ITGB6) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe31-Val992(ITGAV) acidic tail & Gly22-Asn707(ITGB6) basic tail.
- Reinheit
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human ITGAV&ITGB6, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 56.4ng/ml determined by ELISA. See testing image for detail.
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Integrin alpha V beta 6 (ITGAV & ITGB6) Heterodimer (AA 31-992) protein (His-Avi Tag,Biotin)
Spezies: Human Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- Integrin alpha V beta 6 (ITGAV & ITGB6) Heterodimer
- Hintergrund
- ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. ITGAV&ITGB6 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1.
- Molekulargewicht
- 114 kDa (ITGAV)&79.1 kDa (ITGB6). Due to glycosylation, the protein migrates to 90-150 kDa based on Tris-Bis PAGE result.
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