GARP & Latent TGF beta 1 Complex (AA 20-628) protein (His-Avi Tag)

Produktnummer ABIN7274705

Produktdetails

Target: GARP & Latent TGF beta 1 Complex

Protein-Typ: Recombinant

Proteineigenschaft: AA 20-628

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: His-Avi Tag

Verwendungszweck: Human GARP & Latent TGF Beta 1 Complex Protein

Sequenz: His20-Leu628 (GARP) & Leu30-Ser390 (Latent TGF beta 1)

Spezifität: Uni-Prot: Q14392 (GARP), P01137 (Latent TGF beta 1)

Produktmerkmale: Recombinant Human GARP & Latent TGF Beta 1 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His20-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1).

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GARP&Latent TGF Beta 1 Complex at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GARP&Latent TGF Beta 1 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: GARP & Latent TGF beta 1 Complex

Hintergrund: GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.

Molekulargewicht: 70.3 kDa (GARP)&41.4 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 72-80 kDa (GARP)&13 kDa&42-50 kDa (Latent TGF beta 1) based on Tris-Bis PAGE result.

UniProt: Q14392