TNFSF13
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))
Protein-Typ
Recombinant
Proteineigenschaft
AA 112-250, Trimer
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses TNFSF13 Protein ist gelabelt mit His-DYKDDDDK Tag.
Verwendungszweck
Human APRIL/TNFSF13 Trimer Protein
Sequenz
Lys112-Leu250
Produktmerkmale
Recombinant Human APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Lys112-Leu250.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human APRIL Trimer, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Human BCMA, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA. See testing image for detail.
TNFSF13
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
TNFSF13
Spezies: Human
Wirt: Human Cells
Recombinant
> 95 % as determined by reducing SDS-PAGE.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
TNFSF13
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))
The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.
Molekulargewicht
49.9 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.