ENO1 Protein (AA 1-434) (His tag)

Produktnummer ABIN6388125

Produktdetails

Target: ENO1 (Enolase 1 (ENO1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-434

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses ENO1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MGSMSILRIH AREIFDSRGN PTVEVDLYTA KGLFRAAVPS GASTGIYEAL ELRDNDKTRF MGKGVSQAVE HINKTIAPAL VSKKVNVVEQ EKIDKLMIEM DGTENKSKFG ANAILGVSLA VCKAGAVEKG VPLYRHIADL AGNPEVILPV PAFNVINGGS HAGNKLAMQE FMILPVGASS FREAMRIGAE VYHNLKNVIK EKYGKDATNV GDEGGFAPNI LENKEALELL KTAIAKAGYT DQVVIGMDVA ASEFYRSGKY DLDFKSPDDP SRYITPDQLA DLYKSFVQNY PVVSIEDPFD QDDWGAWQKF TASAGIQVVG DDLTVTNPKR IAKAASEKSC NCLLLKVNQI GSVTESLQAC KLAQSNGWGV MVSHRSGETE DTFIADLVVG LCTGQIKTGA PCRSERLAKY NQILRIEEEL GSKAKFAGRS FRNPLAK

Reinheit: > 90 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 6,000 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0 pmole of 2-phosphoglycerate to phosphoenolpyruvate per minute at pH 6.5 at 37C in a couple system with PK and LDH.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-Hcl buffer (  pH 8.5) containing 40 % glycerol, 0.1M NaCl

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: ENO1 (Enolase 1 (ENO1))

Andere Bezeichnung: Eno1 (ENO1 Produkte)

Hintergrund: Eno1 also known as Alpha-Enolase, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of Eno1 is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto's encephalopathy and severe asthma. Recombinant mouse Eno1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Molekulargewicht: 49.5 kDa (457aa) confirmed by MALDI-TOF

NCBI Accession: NP_075608

UniProt: P17182