Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

FAM114A1 Protein (AA 1-569) (Strep Tag)

FAM114A1 Spezies: Maus Wirt: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Produktnummer ABIN3128928
  • Target Alle FAM114A1 Proteine anzeigen
    FAM114A1 (Family with Sequence Similarity 114, Member A1 (FAM114A1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-569
    Spezies
    Maus
    Quelle
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Aufreinigungstag / Konjugat
    Dieses FAM114A1 Protein ist gelabelt mit Strep Tag.
    Applikation
    Western Blotting (WB), ELISA, SDS-PAGE (SDS)
    Sequenz
    MSDDARDTIA NGVGEDAAEM PNSDSPAEDA AEVQCGPATT SNEPAPDDHM EEQPEITAMC AESTPPGILD QSKASAADET PLNGEVTEDT LVECVDSVSL EGDTGSEIPL KEQDDAAVDP SSQAGRWAGW GSWGKSLLSS ASATVGHGLT AVKEKAGATL RIHSANSASP EGAPTDAENG ISGNVTPDQD PARGPHTPPP PGAAGSRGML SALTNVVQNT GKSVLTGGLD ALEFIGKKTM IVLAESDPGF KRTKTLMERT VSLSQMLREA KEKEKQRLAQ QLTVERTAHY GMLFDEYQGL SHLEALEILS NESESKVQSF LTSLDGEKLE LLKNDLISIK DIFVAKESEN EANPEEQGLE ESGEEFARML TELLFELHVA ATPDKLNKAM KKAHDWVEED QSVVSIDVAK GSEEEEKEEG KEEKAEEPEE DKTGGQGAKT VEEVYMLSIE SLAEVTARCI EQLHKVAELI LHGQEEEKPA QDQARVLIKL TTAMCNEVTS LSKKFTNSLT TVGSNKKAEV LNPMINSVFL EGSNSTTYVQ DALQLLLPVL QVSHIRTSCL KATAQPDCS
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Produktmerkmale
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    Expression System:

    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Endotoxin-Niveau
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    Top Product
    Discover our top product FAM114A1 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    FAM114A1 (Family with Sequence Similarity 114, Member A1 (FAM114A1))
    Andere Bezeichnung
    Fam114a1 (FAM114A1 Produkte)
    Synonyme
    Noxp20 Protein, 1190001N04Rik Protein, 9130005N14Rik Protein, family with sequence similarity 114 member A1 Protein, family with sequence similarity 114, member A1 Protein, FAM114A1 Protein, Fam114a1 Protein
    Hintergrund
    Protein Noxp20 (Nervous system overexpressed protein 20) (Protein FAM114A1),FUNCTION: May play a role in neuronal cell development.
    Molekulargewicht
    61.0 kDa
    UniProt
    Q9D281
Sie sind hier:
Kundenservice