IL-33 Protein (AA 112-270)

Produktnummer ABIN1343983

Produktdetails

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 112-270

Spezies: Human, Maus

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Verwendungszweck: IL-33 (human) (rec.) (untagged)

Spezifität: Binds to human ST2.

Kreuzreaktivität: Human, Maus

Produktmerkmale: Human IL-33 (aa 112-270) is untagged.

Reinheit: >95 % (SDS-PAGE)

Endotoxin-Niveau: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Activates human and mouse ST2-dependent NF-kappaB pathway.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 100 μL sterile water.

Konzentration: 0.1 mg/mL

Buffer: Contains PBS.

Handhabung: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Haltbarkeit: 6 months

Referenzen

Suzukawa, Nagase, Ogahara, Han, Tashimo, Shibui, Koketsu, Nakae, Yamaguchi, Ohta: "Leptin enhances survival and induces migration, degranulation, and cytokine synthesis of human basophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 9, pp. 5254-60, (2011) (PubMed).

Suzukawa, Iikura, Koketsu, Nagase, Tamura, Komiya, Nakae, Matsushima, Ohta, Yamamoto, Yamaguchi: "An IL-1 cytokine member, IL-33, induces human basophil activation via its ST2 receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 9, pp. 5981-9, (2008) (PubMed).

Antigendetails

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Andere Bezeichnung: IL-33 (IL33 Produkte)

Hintergrund:

Interleukin-33, IL-1F11, NF-HEV

Interleukin-33 (IL-33, HF-NEV, IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released upon cell lysis. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1(MAPK3)/ERK2(MAPK1), p38(MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.

Molekulargewicht: ~18kDa

UniProt: Q2YEJ5

Pathways: Production of Molecular Mediator of Immune Response