Antigen 85-A protein (His tag)

Produktnummer ABIN1098251

Produktdetails

Target: Antigen 85-A

Protein-Typ: Recombinant

Spezies: Mycobacterium tuberculosis

Quelle: Hi-5 Cells

Aufreinigungstag / Konjugat: His tag

Applikation: SDS-PAGE (SDS)

Aufreinigung: purified by chromatography

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Kommentare:

Synonyms: Antigen 85 complex A, Mycolytransferase 85A, Fibronectin-binding protein A, TB Ab85A, TBab85A

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol

Lagerung: 4 °C

Informationen zur Lagerung: Avoid repeated freezing and thawing cycles.

Antigendetails

Target: Antigen 85-A

Andere Bezeichnung: Antigen 85A

Hintergrund: Antigen 85A, belong to the antigen 85 complex (Antigen 85A, B, C). The enzymes of the antigen 85 complex possess mycolyltransferase activity and catalyze the synthesis of the most abundant glycolipid of the mycobacterial cell wall, the cord factor. The cord factor (trehalose 6,6'-dimycolate, TDM) is essential for the integrity of the mycobacterial cell wall and pathogenesis of the bacillus. TDM is synthesized from two molecules of trehalose-6'-monomycolate (TMM) by Antigen 85A. Recombinant Mycobacterium tuberculosis Antigen 85A protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell and purified by using conventional chromatography techniques.

Molekulargewicht: 32.8kDa (305aa)

NCBI Accession: NP_218321