This is a synthetic peptide designed for use in combination with anti-Bmi1 Antibody. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
Each Investigator should determine their own optimal working dilution for specific applications.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
Konzentration
1 mg/mL
Buffer
Final peptide concentration is 1 mg/mL in PBS.
Handhabung
Avoid repeated freeze-thaw cycles.
Lagerung
-20 °C
Informationen zur Lagerung
For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
Target
BMI1
(BMI1 Polycomb Ring Finger Oncogene (BMI1))
Hintergrund
Bmi1 is a component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Alias Symbols: AW546694, Bmi-1, Pcgf4
Protein Interaction Partner: Phc2,Rnf2,CBX4,CBX8,Cbx2,Dmap1,Dnmt3b,HSPA4,Kdm2b,PHC1,PHC2,PHC3,Phc1,Phc3,RING1,Rnf2,SCMH1,SMARCA5,Slc11a1