When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
NCAPH
(Non-SMC Condensin I Complex, Subunit H (NCAPH))
Synonyme
NCAPH Peptide, si:dkeyp-86b9.4 Peptide, zgc:158618 Peptide, BRRN1 Peptide, CAP-H Peptide, brrn1 Peptide, cap-h Peptide, A730011O11Rik Peptide, Brrn1 Peptide, HCAP-H Peptide, mKIAA0074 Peptide, non-SMC condensin I complex subunit H Peptide, non-SMC condensin I complex, subunit H Peptide, condensin complex subunit 2 Peptide, non-SMC condensin I complex subunit H S homeolog Peptide, NCAPH Peptide, ncaph Peptide, LOC708975 Peptide, ncaph.S Peptide, Ncaph Peptide