SOD Assay Kit

Produktnummer ABIN955780

Produktdetails

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Nachweismethode: Colorimetric

Bestandteile: 1 x 5 mL WST Solution
1 x 100 µL Enzyme Solution
1 x 100 mL Buffer Solution
1 x 50 mL Dilution Buffer.

Benötigtes Material: Plate reader (450 nm filter).
96-well microplate.
2-20 µL & 20-200 µL pipettes and a multi-channel pipette.
Incubator.

Anwendungsinformationen

Ergebnisberechnung:

1. Add 20 µL of sample solution to each sample and blank 2 well, and add 20 µL of ddH2O (double distilled water) to each blank 1 and blank 3 well.
   2. Add 200 µL of WST Working Solution to each well, and mix.
   3. Add 20 µL of Dilution Buffer to each blank 2 and blank 3 well.
   4. Add 20 µL of Enzyme Working Solution to each sample and blank 1 well, and then mix thoroughly.
   Since superoxide will be released immediately after the addition of Enzyme Working Solution to a well, use a multi-channel pipette to avoid the reaction time lag of each well. Inhibition activity can also be determined by a kinetic method. Please determine an incubation time range that has a linearity of the slope before the assay. A good linearity should be observed up to 20 min.
   5. Incubate the plate at 37°C for 20 min.
   6. Read the absorbance at 450 nm using a microplate reader.
   7. Calculate the SOD activity (inhibition rate %)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Please store at 4°C. Protect the WST Solution and WST Working Solution from light.

Antigendetails

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Andere Bezeichnung: SOD (SOD1 Produkte)

Hintergrund: Superoxide Dismutase (SOD), which catalyzes the dismutation of the superoxide anion (O2 - ) into hydrogen peroxide and molecular oxygen, is one of the most important antioxidative enzymes. In order to determine the SOD activity, several direct and indirect methods have been developed. Among these methods, an indirect method using nitroblue tetrazolium (NBT) is commonly used due to its convenience and ease of use. However, there are several disadvantages to the NBT method, such as poor water solubility of the formazan dye and the interaction with the reduced form of xanthine oxidase. The SOD Assay allows very convenient SOD assaying by utilizing 's highly water- soluble tetrazolium salt, WST-1 (2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt) that produces a water-soluble formazan dye upon reduction with a superoxide anion. The rate of the reduction with O2 - are linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD. Therefore, the IC50 (50% inhibition activity of SOD or SOD-like materials) can be determined by a colorimetric method.