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EpCAM ELISA Kit (Epithelial Cell Adhesion Molecule) ELISA Kit

EPCAM Reaktivität: Human AA 24-265 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Produktnummer ABIN2859292
$369.00
Zzgl. Versandkosten $45.00
96 tests
Lieferung in 4 bis 6 Werktagen
  • Target Alle EpCAM (EPCAM) ELISA Kits anzeigen
    EpCAM (EPCAM)
    Bindungsspezifität
    AA 24-265
    Reaktivität
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    93.7-6000 pg/mL
    Untere Nachweisgrenze
    93.7 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human EPCAM
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: Q24 - K265
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: Q24-K265
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Belongs to the EPCAM family. Contains 1 thyroglobulin type-1 domain.

    Tissue Specificity: Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .

    Plattentyp
    Pre-coated
    Protokoll
    human EPCAM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EPCAM has been precoated onto 96-well plates. Standards(NSO, Q24 - K265) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPCAM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human EPCAM amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/m human EPCAM standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human EPCAM standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 475, Standard deviation: 16.15, CV(%): 3.4
    • Sample 2: n=16, Mean(pg/ml): 1837, Standard deviation: 77.15, CV(%): 4.2
    • Sample 3: n=16, Mean(pg/ml): 3642, Standard deviation: 193.03, CV(%): 5.3,
    • Sample 1: n=24, Mean(pg/ml): 531, Standard deviation: 24.43, CV(%): 4.6
    • Sample 2: n=24, Mean(pg/ml): 2400, Standard deviation: 136.8, CV(%): 5.7
    • Sample 3: n=24, Mean(pg/ml): 4583, Standard deviation: 297.9, CV(%): 6.5
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle EpCAM (EPCAM) ELISA Kits anzeigen
    EpCAM (EPCAM)
    Andere Bezeichnung
    EPCAM (EPCAM Produkte)
    Synonyme
    DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, TACSTD1, TROP1, ECS-1, ECS1, ESA1, M17S1, EGP, Ep-CAM, sb:cb6, tacstd, wu:fj17g02, zgc:110304, zgc:77119, tacstd1, MGC80540, EPCAM, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2, CD326, Egp314, EpCAM1, GA733-2, Ly74, Tacsd1, Tacstd1, gp40, epithelial cell adhesion molecule, flotillin 2, epithelial cell adhesion molecule S homeolog, EPCAM, FLOT2, epcam, epcam.S, Epcam
    Hintergrund

    Protein Function: May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. .

    Background: Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. Also it is involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since it is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies.

    Synonyms: Epithelial cell adhesion molecule,Ep-CAM,Adenocarcinoma-associated antigen,Cell surface glycoprotein Trop-1,Epithelial cell surface antigen,Epithelial glycoprotein,EGP,Epithelial glycoprotein 314,EGP314,hEGP314,KS 1/4 antigen,KSA,Major gastrointestinal tumor-associated protein GA733-2,Tumor-associated calcium signal transducer 1,CD326,EPCAM,GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1,

    Full Gene Name: Epithelial cell adhesion molecule

    Cellular Localisation: Lateral cell membrane, Single-pass type I membrane protein . Cell junction, tight junction . Colocalizes with CLDN7 at the lateral cell membrane and tight junction..
    Gen-ID
    4072
    UniProt
    P16422
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