Defensin, beta 103B (DEFB103B) ELISA Kit

Details zu Produkt Nr. ABIN2043301
Target
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
3
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
31.25-2000 pg/mL
Untere Nachweisgrenze
31.25 pg/mL
Applikation
ELISA
Optionen
Marke BioAssay™
Nachweismethode Colorimetric
Sensitivität < 12.7 pg/mL
Target
Andere Bezeichnung Defensin beta 103B (DEFB103B) (DEFB103B ELISA Kit Abstract)
Pathways Production of Molecular Mediator of Immune Response
Plattentyp Pre-coated
Protokoll Defensin Beta 103B (DEFB103B) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 103B (DEFB103B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 103B (DEFB103B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 103B (DEFB103B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Defensin Beta 103B (DEFB103B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Beschränkungen Nur für Forschungszwecke einsetzbar
Lagerung 4 °C/-20 °C