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Endothelin 1 ELISA Kit

EDN1 Reaktivität: Human AA 53-73 Colorimetric Sandwich ELISA 3.9-250 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Pubmed (2 Referenzen)
Produktnummer ABIN1672856
$369.00
Zzgl. Versandkosten $45.00
96 tests
Lieferung in 4 bis 6 Werktagen
  • Target Alle Endothelin 1 (EDN1) ELISA Kits anzeigen
    Endothelin 1 (EDN1)
    Bindungsspezifität
    AA 53-73
    Reaktivität
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    3.9-250 pg/mL
    Untere Nachweisgrenze
    3.9 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Endothelin
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: human peptide
    Immunogen sequence: C53-W73
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Homologie
    Hamster
    Sensitivität
    <0.5pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: human peptide
    Immunogen sequence: C53-W73
    Featured
    Zu unserem meistverkauften EDN1 ELISA Kit
  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Belongs to the endothelin/sarafotoxin family.

    Tissue Specificity: Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. .

    Plattentyp
    Pre-coated
    Protokoll
    human Endothelin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Endothelin has been precoated onto 96-well plates. Standards(human peptide, C53-W73) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Endothelin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Endothelin amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 16.5pg/mL, 7.8pg/mL, 3.9pg/mL human Endothelin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Endothelin standard solution and each sample is measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 25.1, Standard deviation: 1.41, CV(%): 5.6
    • Sample 2: n=16, Mean(pg/ml): 85.4, Standard deviation: 3.76, CV(%): 4.4
    • Sample 3: n=16, Mean(pg/ml): 146, Standard deviation: 8.03, CV(%): 5.5,
    • Sample 1: n=24, Mean(pg/ml): 23.2, Standard deviation: 1.79, CV(%): 7.7
    • Sample 2: n=24, Mean(pg/ml): 84.3, Standard deviation: 6.07, CV(%): 7.2
    • Sample 3: n=24, Mean(pg/ml): 143, Standard deviation: 8.72, CV(%): 6.1
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Liu, Zhu, Zhou, Wei, Long, Chen, Ling, Ge, Zhuo: "Endoplasmic reticulum stress promotes amyloid-beta peptides production in RGC-5 cells." in: Cell stress & chaperones, Vol. 19, Issue 6, pp. 827-35, (2014) (PubMed).

    Peng, Dai, Ji, Dai: "The separate roles of endothelin receptors participate in remodeling of matrix metalloproteinase and connexin 43 of cardiac fibroblasts in maladaptive response to isoproterenol." in: European journal of pharmacology, Vol. 634, Issue 1-3, pp. 101-6, (2010) (PubMed).

  • Target Alle Endothelin 1 (EDN1) ELISA Kits anzeigen
    Endothelin 1 (EDN1)
    Andere Bezeichnung
    EDN1 (EDN1 Produkte)
    Synonyme
    et1, et-1, Edn1, EDN, ET-1, ET1, PPET1, HDLCQ7, preproET, Et1, fb14d01, wu:fb14d01, PPET-1, EDN1, edn1, edn1-a, edn1-b, endothelin 1, endothelin 1 S homeolog, endothelin 1 L homeolog, edn1, EDN1, Edn1, edn1.S, edn1.L
    Hintergrund

    Protein Function: Endothelins are endothelium-derived vasoconstrictor peptides.

    Background: The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure(hypertension) and heart disease.

    Synonyms: Endothelin-1,Preproendothelin-1,PPET1,Endothelin-1,ET-1,Big endothelin-1,EDN1,

    Full Gene Name: Endothelin-1

    Cellular Localisation: Secreted.
    Gen-ID
    1906
    UniProt
    P05305
    Pathways
    Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size
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