TagFP Selector

Details zu Produkt Nr. ABIN5311511, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Entacmaea quadricolor
Wirt
Camelid (Camelidae)
Applikation
Protein Complex Immunoprecipitation (Co-IP), Immunoprecipitation (IP), Purification (Purif), Chromatin Immunoprecipitation (ChIP), RNA-Binding Protein Immunoprecipitation (RIP)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Proben Cell Extracts
Spezifität The Antibody with Clone 1H7 recognizes mTagBFP, mTagRFP, mTagRFP657 and mKate. Reactivity against other TagRFP derivatives not analyzed. Does not cross-react with common GFP- or dsRed/RFP derivatives including EBFP.
Produktmerkmale BFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4 % cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. TagFP Selector thus features high affinity and superior capacity for TagFP fusion proteins while showing negligible non-specific background. TagFP Selector is compatible not only with physiological buffers but also with high stringency buffers. BFP Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.
Bestandteile 4 % cross-linked agarose (bead size 50-150 μm) with covalently immobilized single-domain antibody
Benötigtes Material wash buffers, columns, tubes
Bead Ligand Antibody
Bead Matrix Agarose beads
Bead Size 90 μm
Hintergrund Blue fluorescent protein BFP and its derivates have become very popular and universal tools in cell biology. It is a monomeric protein and an excellent fusion partner for chimeric proteins. The possibility to fuse BFP to the N- or the C-terminus of the pr
Applikationshinweise 200 μL slurry for up to 10 reactions
Kommentare

Capacity: > 4 μg BFP per μl of packed beads (= 2μl of slurry)

Beschränkungen Nur für Forschungszwecke einsetzbar
Buffer 50 % slurry in 20 % ethanol / PBS 0.09 % sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Bilder des Herstellers
Immunoprecipitation (IP) image for TagFP Selector
 (ABIN5311511) A) Pull-down of mTagBFP from a mixture of GFP, mCherry and mTagBFP B) Immunoprecipit...