Casein Kinase II alpha /alpha (AA 1-123) Antikörper

Details zu Produkt Nr. ABIN968609, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
AA 1-123
1
Reaktivität
Human, Maus, Ratte (Rattus)
1
1
1
Wirt
Maus
1
Klonalität (Klon)
Monoklonal ()
Applikation
Flow Cytometry (FACS), Western Blotting (WB)
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Rat Casein Kinase II alpha aa. 1-123
Klon 31-Casein Kinase IIalpha-alpha'
Isotyp IgG1
Kreuzreaktivität Human, Maus
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Hintergrund Casein kinase II (CKII, CK2) is a pleiotropic, ubiquitous, and constitutively active Ser/Thr kinase that utilizes both ATP and GTP as phosphoryl donors to catalyze the phosphorylation of numerous protein substrates. It has been implicated in DNA replication, control of metabolism, and regulation of transcription and translation. CKII is a messenger independent kinase due to the fact that its activity is not affected by factors that regulate other protein kinases (e.g. cAMP, Ca2+, diacylglycerol). It is composed of two catalytic (alpha and alpha') subunits and two regulatory (beta) subunits. The beta subunits stabilize the holoenzyme and enhance the activity of the alpha subunits. Although the enzyme exists as a tetramer, the free alpha/alpha' subunits are catalytically active by themselves and are present in cells under some conditions. The alpha and alpha' subunits (85% homologous) are encoded by distinct genes and have been detected in multiple species including human, bovine, and avian. CKII is found primarily in the nucleus in dividing cells, but can be detected in the cytoplasm in quiescent cells. Thus, CKII activity is required for cell cycle progression and other essential signal transduction events.
Molekulargewicht 45 kDa
Kommentare

Related Products: ABIN968546, ABIN967389

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store undiluted at -20°C.
Bilder des Herstellers
Western Blotting (WB) image for anti-Casein Kinase II alpha /alpha (AA 1-123) antibody (ABIN968609) Western blot analysis of Casein Kinase alpha/alpha' on rat cerebellum lysate. Lane 1:...
Produkt verwendet in: Freeman, Brebner, Lynch, Patel, Robertson, Roberts, Vrana: "Changes in rat frontal cortex gene expression following chronic cocaine." in: Brain research. Molecular brain research, Vol. 104, Issue 1, pp. 11-20, 2002 (PubMed).

Greer, Wang, Raman, Justement: "CD45 function is regulated by an acidic 19-amino acid insert in domain II that serves as a binding and phosphoacceptor site for casein kinase 2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 12, pp. 7208-18, 2001 (PubMed).

Xu, Toselli, Russell, Seldin: "Globozoospermia in mice lacking the casein kinase II alpha' catalytic subunit." in: Nature genetics, Vol. 23, Issue 1, pp. 118-21, 1999 (PubMed).

Kikkawa, Mann, Firtel, Hunter: "Molecular cloning of casein kinase II alpha subunit from Dictyostelium discoideum and its expression in the life cycle." in: Molecular and cellular biology, Vol. 12, Issue 12, pp. 5711-23, 1992 (PubMed).

Lozeman, Litchfield, Piening, Takio, Walsh, Krebs: "Isolation and characterization of human cDNA clones encoding the alpha and the alpha' subunits of casein kinase II." in: Biochemistry, Vol. 29, Issue 36, pp. 8436-47, 1991 (PubMed).

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