CA2 Antikörper

Produktnummer ABIN96020

Hersteller Produkt- Nr.: 100-4157

Der Kaninchen Polyklonal Anti-CA2-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, CA2 in Proben von Rind (Kuh) zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für CA2 Antikörper (ABIN96020)

Target: CA2 (Carbonic Anhydrase II (CA2))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-CA2 Antikörper

Hersteller Produkt- Nr.: 100-4157

Hersteller: Rockland

Verwendungszweck: Carbonic Anhydrase II Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes].

Produktmerkmale: Synonyms: rabbit anti-Carbonic Anhydrase II Antibody, Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody

Aufreinigung: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Carbonic Anhydrase II [Bovine Erythrocytes]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise:

Application Note: This product has been assayed against 1.0 μg of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3,000 to 1:14,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 85 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-CA2 Antikörper (ABIN96020)

Loussert-Fonta, Toullec, Paraecattil, Jeangros, Krueger, Escrig, Meibom: "Correlation of fluorescence microscopy, electron microscopy, and NanoSIMS stable isotope imaging on a single tissue section." in: Communications biology, Vol. 3, Issue 1, pp. 362, (2020) (PubMed).

Details zu CA2

Target: CA2 (Carbonic Anhydrase II (CA2))

Andere Bezeichnung: CA2

Hintergrund: Background: Carbonic Anhydrase 2 is essential for bone resorption and osteoclast differentiation. It reverses hydration of carbon dioxide and can hydrate cyanamide to urea. It is involved in the regulation of fluid secretion into the anterior chamber of the eye. Carbonic Anhydrase II contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. It stimulates the chloride-bicarbonate exchange activity of SLC26A6. it is used for target of drugs used in treatments against glaucoma disorder and breast cancer.

Gen-ID: 280740

NCBI Accession: NP_848667

UniProt: P00921