beta Amyloid Antikörper (Amyloid beta) (N-Term)

Details for Product anti-Abeta Antibody No. ABIN95037
Antigen
  • AAA
  • ABETA
  • ABPP
  • AD1
  • APPI
  • CTFgamma
  • CVAP
  • PN-II
  • PN2
  • aaa
  • abeta
  • abpp
  • ad1
  • appi
  • ctfgamma
  • cvap
  • pn2
  • Abeta
  • Abpp
  • Adap
  • Ag
  • Cvap
  • E030013M08Rik
  • betaApp
  • amyloid beta precursor protein
  • amyloid beta (A4) precursor protein
  • APP
  • app
  • App
Epitop
N-Term
52
35
20
10
10
7
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
164
39
27
9
6
5
5
4
3
3
2
2
1
1
1
1
1
Wirt
Kaninchen
105
67
6
2
Klonalität
Polyklonal
Konjugat
Dieser beta Amyloid Antikörper ist unkonjugiert
11
4
2
1
1
1
Applikation
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
116
98
59
40
36
29
17
17
11
5
5
3
2
2
1
1
1
1
1
1
1
Optionen
'Independent Validation' Siegel
  • Erfolgreich validiert (Unfolding Profile)
  • durch  NanoTemper Technologies
  • Nr. #104082
  • Datum 23.07.2019
  • This product was validated with a different quantity.
Antigen ABETA
Validierte Anwendung Unfolding Profile
Positivkontrolle ABIN1043866
Bewertung

Passed. ABIN1043866 showed Ti at 83.8°C and a clear unfolding profile with one unfolding event. This suggests that the antibody is properly folded and functional.

Protokoll
  • Load the undiluted sample into Tycho capillary (NanoTemper Technologies, TY-C001).
  • Run Tycho measurement.
Anmerkungen

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Validierungsbilder
Unfolding Profile validation image for anti-Amyloid beta (Abeta) (N-Term) antibody (ABIN1043866) Unfolding profile of ABIN1043866. The fluorescence signal is plotted against temperat...
Immunogen This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the amino terminus (aa 1-14) of human beta amyloid conjugated to KLH using maleimide.
Immunogen Type: Peptide
Isotyp IgG
Spezifität This affinity-purified antibody is directed against the amino terminal end of beta amyloid and is useful in determining its presence in various assays. Polyclonal anti-beta amyloid detects human and mouse beta amyloid. Cross reactivity with beta amyloid from other species is likely but has not been determined.
Produktmerkmale Beta amyloid, often abbreviated as A-beta, is a protein that builds up in the brains of persons with Alzheimer's disease, collecting in clumps called plaques or senile plaques. While some researchers question whether beta amyloid is the cause of the dementia, most agree that it is involved in the disruption of thinking that is a hallmark of the disease. In some cases of familial Alzheimer's disease, mutations in genes for the proteins called the presenilins lead to increased production of amyloid. Researchers have been looking at how presenilin-1 in particular contributes to the excess buildup of beta amyloid. Presenilin-1 apparently acts to increase the activity of gamma-secretase, an enzyme that changes a normal protein (amyloid precursor protein or APP) into beta amyloid itself. Furthermore, presenilin-1 might be gamma-secretase.
Reinigung affinity purified
Sterilität Sterile filtered
Antigen
Andere Bezeichnung Beta Amyloid (Abeta Antibody Abstract)
Hintergrund Beta amyloid, often abbreviated as A-beta, is a protein that builds up in the brains of persons with Alzheimer's disease, collecting in clumps called plaques or senile plaques. While some researchers question whether beta amyloid is the cause of the dementia, most agree that it is involved in the disruption of thinking that is a hallmark of the disease. In some cases of familial Alzheimer's disease, mutations in genes for the proteins called the presenilins lead to increased production of amyloid. Researchers have been looking at how presenilin-1 in particular contributes to the excess buildup of beta amyloid. Presenilin-1 apparently acts to increase the activity of gamma-secretase, an enzyme that changes a normal protein (amyloid precursor protein or APP) into beta amyloid itself. Furthermore, presenilin-1 might be gamma-secretase.
Synonyms: Beta amyloid, A-beta
Gen-ID 351
NCBI Accession NP_000475
UniProt P05067
Applikations-hinweise Affinity purified anti-beta amyloid detects beta amyloid in ELISA, IHC and IF. A 40-50 kD band consistent with a higher MW precursor is detected in western blot using whole tissue extracts from mouse brain. In general, we recommend the use of 4% PFA for paraffin embedded tissues and 10% formalin for frozen tissue for fixation.
Kommentare

Gene Name: APP

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Haltbarkeit 12 months
Bilder des Herstellers
 image for anti-Amyloid beta (Abeta) (N-Term) antibody (ABIN95037) anti-Amyloid beta (Abeta) (N-Term) antibody
Immunohistochemistry (IHC) image for anti-Amyloid beta (Abeta) (N-Term) antibody (ABIN95037) Immunohistochemistry with anti-beta amyloid antibody showing amyloid beta plaque stai...
 image for anti-Amyloid beta (Abeta) (N-Term) antibody (ABIN95037) Human Heart (formalin-fixed, paraffin-embedded) stained with Anti-Beta Amyloid Antibo...
Western Blotting (WB) image for anti-Amyloid beta (Abeta) (N-Term) antibody (ABIN95037) Western Blot of Rabbit anti-Beta Amyloid Antibody. Lane 1: HEK293 WCL. Lane 2: Mouse ...
ELISA image for anti-Amyloid beta (Abeta) (N-Term) antibody (ABIN95037) ELISA results of purified Rabbit anti-Beta Amyloid Antibody tested against BSA-conjug...
Western Blotting (WB) image for anti-Amyloid beta (Abeta) (N-Term) antibody (ABIN95037) Western Blot of Rabbit anti-Beta Amyloid antibody. Marker: Opal Pre-stained ladder . ...
Immunofluorescence (IF) image for anti-Amyloid beta (Abeta) (N-Term) antibody (ABIN95037) Immunofluorescence microscopy of Rabbit Anti-Beta Amyloid antibody using HeLa cells f...
Produkt verwendet in: Frenkel, Balass, Solomon: "N-terminal EFRH sequence of Alzheimer's beta-amyloid peptide represents the epitope of its anti-aggregating antibodies." in: Journal of neuroimmunology, Vol. 88, Issue 1-2, pp. 85-90, 1998 (PubMed).

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