Rekombinanter T Cell Receptor Antikörper
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- Target
- T Cell Receptor
- Antikörpertyp
- Recombinant Antibody
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Reaktivität
- Maus
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Wirt
- Kaninchen
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Klonalität
- Chimeric
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Konjugat
- Unkonjugiert
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Applikation
- Flow Cytometry (FACS), Immunoprecipitation (IP), Crystallization (Crys), Immunofluorescence (IF)
- Verwendungszweck
- Anti-TCR [Desire-1], Rabbit IgG, kappa
- Spezifität
- MAb desire-1 recognises an epitope contributed by both the alpha and beta chains of the KB5-C20 TCR.
- Produktmerkmale
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Original Species of Ab: Mouse
Original Format of Ab: IgG
- Aufreinigung
- Protein A affinity purified
- Immunogen
- mAb desire-1 was obtained after fusion of spleen cells from a BALB.K mouse immunised twice with KB5-C20 clone cells in RPMI 1640 medium, with non-Ig-producing myeloma cells X63-Ag8.653. Desire-1 was selected for its capacity to inhibit selectively the H-2Kb-specific cytolysis by clone KB5-C20.
- Klon
- Desire-1
- Isotyp
- IgG kappa
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- Applikationshinweise
- Desire-1 has been used for the immunoprecipitation of soluble TCR molecules (Gregoire 1991 & Hua 1986). An FITC conjugated desire-1 has been used in flow cytometric analysis as a marker for clonotype expression (Schonrich 1991). Further, a Fab fragment derived from desire-1 has been used in the crystallisation of the T-cell receptor for 3D structure determination (Housset 1997).
- Kommentare
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1 mg/mL
- Buffer
- PBS with 0.02 % Proclin 300.
- Konservierungsmittel
- ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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