Syntaxin 4 Antikörper (Intracellular)

Produktnummer ABIN7043796

Produktdetails anti-Syntaxin 4 Antikörper

Target: Syntaxin 4 (STX4)

Bindungsspezifität: AA 2-23, Intracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Syntaxin 4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Verwendungszweck: A Rabbit Polyclonal Antibody to Syntaxin 4

Spezifität: Intracellular, N-terminus

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse - identical, human - 17,22 amino acid residues identical

Produktmerkmale: Anti-Syntaxin 4 Antibody is directed against an epitope of rat Syntaxin 4. Anti-Syntaxin 4 Antibody (ABIN7043796, ABIN7044650 and ABIN7044651)) can be used in western blot and immunohistochemical applications. It was designed to recognize Syntaxin 4 from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: RDRTHELRQGDNISDDEDEVRV(C), corresponding to residues 2-23 of rat syntaxin 4

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:400

Kommentare:

Negative Control: BLP-NR004

Blocking Peptide: BLP-NR004

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Syntaxin 4

Target: Syntaxin 4 (STX4)

Andere Bezeichnung: STX4 (STX4 Produkte)

Hintergrund: STX4, Renal carcinoma antigen NY-REN-31, P35-2,Syntaxin 4 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60-70 amino acids that are arranged in heptad repeats1,2.SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.Syntaxin 4, like most SNAREs, is a type IV membrane protein with a relatively large N-terminus containing the SNARE motif located in the cytoplasmic side and a transmembrane domain located close to the C-terminus that functions as an anchor1,2.Syntaxin 4 functions as a plasma membrane protein t-SNARE and has been shown to have an important role in pancreatic b-cell insulin secretion and in insulin-stimulated glucose uptake in skeletal muscle and adipose tissue by means of translocation of the glucose transporter GLUT4 from an intracellular compartment to the plasma membrane3,4.

Alternative names: Syntaxin 4, STX4, Renal carcinoma antigen NY-REN-31, P35-2

Gen-ID: 81803

NCBI Accession: NM_004604

UniProt: Q08850

Pathways: Synaptic Vesicle Exocytosis