STOML1 Antikörper (Extracellular)

Produktnummer ABIN7043778

Produktdetails anti-STOML1 Antikörper

Target: STOML1 (Stomatin (EPB72)-Like 1 (STOML1))

Bindungsspezifität: AA 4-17, Extracellular

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser STOML1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: A Rabbit Polyclonal Antibody to Stomatin-Like Protein 1

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,human - identical

Produktmerkmale: Anti-Stomatin-like Protein 1 (extracellular) Antibody (ABIN7043778, ABIN7044516 and ABIN7044517)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize STOML1 from human, mouse, and rat samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RSGYRALPLGDFDR, corresponding to amino acid residues 4-17 of mouse STOML1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:500-1:800

Kommentare:

Negative Control: BLP-IP001

Blocking Peptide: BLP-IP001

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu STOML1

Target: STOML1 (Stomatin (EPB72)-Like 1 (STOML1))

Andere Bezeichnung: STOML1 (STOML1 Produkte)

Hintergrund: STOML1, SLP-1, STORP, Slipin-1, hUNC-24,The Stomatin-like protein family contains five members and are characterized by the presence of a structurally conserved core called the stomatin-domain. This stomatin-domain is further related to a domain found in members of the prohibitin, flotillin and HflK/HflC protein families and has accordingly also been called the SPFH domain.All mammalian stomatin proteins are integral membrane protein with a single, relatively short, hydrophobic membrane insertion domain, followed by the core stomatin-domain. A single conserved proline residue (known to be a potent helix breaker) in the hydrophobic region is required for the formation of the protein's hairpin structure. The N-terminal domain is extracellular. Stomatin-like protein 1 (STOML1) has a typical stomatin-domain at the N-terminus and an additional sterol carrier protein-2 (SCP-2) domain at the C-terminus. STOML-1 is targeted to the late endosomal compartment where it interacts with stomatin. STOML-1 is predominantly expressed in the brain, in a subpopulation of sensory neurons in the dorsal root ganglia. Much lower levels are detected in other tissues1.Following anoxia, STOML-1 expression is up-regulated by the oxidative response factor SKN-1/Nrf. SKN-1/Nrf is required for both rapid mitochondrial refusion and rapid behavioral recovery during reoxygenation suggesting that STOML-1 helps facilitate mitochondrial dynamics following anoxia2.STOML-1 also modulates the proton-sensitive members of the acid-sensing ion channel (ASIC) family. STOML-1 strongly inhibits ASIC1a and accelerates the inactivation time constant of ASIC3, but has no effect on the splice variant ASIC1b3.

Alternative names: Stomatin-like Protein 1, STOML1, SLP-1, STORP, Slipin-1, hUNC-24

Gen-ID: 69106

NCBI Accession: NM_004809

UniProt: Q8CI66