Prostacyclin Receptor Antikörper (Extracellular)

Produktnummer ABIN7043563

Produktdetails anti-Prostacyclin Receptor Antikörper

Target: Prostacyclin Receptor (PTGIR)

Bindungsspezifität: AA 290-302, Extracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Prostacyclin Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Verwendungszweck: A Rabbit Polyclonal Antibody to Prostacyclin Receptor (PTGIR)

Spezifität: 3rd extracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse - identical, human - 11,13 amino acid residues identical

Produktmerkmale: Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody is directed against an epitope of rat the PGI2 receptor. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody (ABIN7043563, ABIN7045122 and ABIN7045123)) can be used in western blot analysis. The antibody recognizes an extracellular epitope, ideal for detecting the receptor in living cells. It has been designed to recognize PGI2 receptor from human, mouse and rat samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)TQAIAPDSREMGD, corresponding to amino acid residues 290-302 of rat PTGIR

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:400

Kommentare:

Negative Control: BLP-PR068

Blocking Peptide: BLP-PR068

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Prostacyclin Receptor

Target: Prostacyclin Receptor (PTGIR)

Andere Bezeichnung: PTGIR (PTGIR Produkte)

Hintergrund: Prostanoid IP receptor, Prostaglandin I2 receptor, PGI2 receptor,Prostaglandins comprise a family of locally acting hormones, whose synthesis is initiated by the enzyme COX. This pathway is involved in a broad variety of pathological conditions such as cancer, inflammation and hypertension.Prostaglandin I2 receptor (PGI2 receptor) is G-coupled protein receptor (GPCR) with seven hydrophobic transmembrane segments and an extracellular NH2 terminus. Prostaglandin receptors belong to family A, group V of GPCRs. There is significant ligand-binding cross-reactivity between different prostaglandins and different receptors in the family. Within the PG family, receptors share roughly 20-30 % of their sequence and there are 34 identical residues across the family. Twenty of these residues are unique to PG receptors and are not shared by other GPCRs. The unique residues reside mostly in the transmembrane region and in the second extracellular loop region. Both of these regions are implicated in ligand binding. The PGI2 receptor signals primarily via stimulation of cAMP generation but through PIP2 as well despite a much higher agonist concentration required for PIP2 hydrolysis.PGI2 receptor mRNA is highly expressed in the human heart, aorta, kidney, liver and lung tissues and in mice thymus, heart and spleen1. Mice lacking PGI2 receptor have been shown to have a reduced inflammation response and a significant decrease in clinical arthritis. In addition, human patients with a dysfunctional PGI2 receptor variant exhibit an enhanced atherothrombotic phenotype with higher incidence of triple vessel coronary disease and cardiovascular events2.

Alternative names: Prostacyclin Receptor (PTGIR), Prostanoid IP receptor, Prostaglandin I2 receptor, PGI2 receptor

Gen-ID: 292661

NCBI Accession: NM_000960

UniProt: P43253

Pathways: cAMP Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling