HRH1 Antikörper (AA 396-409)

Produktnummer ABIN7043268

Produktdetails anti-HRH1 Antikörper

Target: HRH1 (Histamine Receptor H1 (HRH1))

Bindungsspezifität: AA 396-409

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HRH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Immunochromatography (IC)

Verwendungszweck: A Rabbit Polyclonal Antibody to Histamine H1 Receptor

Spezifität: 3rd intracellular loop

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse - identical, human,dog - 13,14 amino acid residues identical

Produktmerkmale: Anti-Histamine H1 Receptor (HRH1) Antibody (ABIN7043268, ABIN7044505 and ABIN7044506)) is a highly specific antibody directed against an epitope of rat H1R. The antibody can be used in western blot, immunocytochemistry, indirect flow cytometry, and immunohistochemstry applications. It has been designed to recognize H1R from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HSRQYVSGLHLNRE, corresponding to amino acids 396-409 of rat HRH1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:400

Kommentare:

Cited Application: IHC|IFC

Negative Control: BLP-HR001

Blocking Peptide: BLP-HR001

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu HRH1

Target: HRH1 (Histamine Receptor H1 (HRH1))

Andere Bezeichnung: HRH1 (HRH1 Produkte)

Hintergrund: H1R, HH1R,Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1The biological effects of histamine are mediated through four receptors (H1-H4 receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.H1 receptors couple to Gq/G11 proteins leading to phospholipase C activation, inositol phosphate production and calcium mobilization.1H1 receptors are expressed in many cell types including endothelial and smooth muscle cells where they mediate vasodilatation and increased vascular permeability effects. H1R is widely distributed through the brain where it mediates sleep-wake cycles and regulates cognitive functions. The receptor is expressed in several cell populations involved in the immune response, including T-cells and B-cells, monocytes and eosinophiles.2,3H1 anti-histamine compounds have been available for almost thirty years and are among the most commonly used medication for conditions such as allergic inflammation. In addition, H1 anti-histamines are also prescribed for the treatment of insomnia and analgesia.2,3

Alternative names: Histamine H1 Receptor (HRH1), H1R, HH1R

Gen-ID: 24448

NCBI Accession: NM_032369

UniProt: P31390

Pathways: Regulation of Carbohydrate Metabolic Process