ChT Antikörper (Extracellular)

Produktnummer ABIN7043267

Der Kaninchen Polyklonal Anti-ChT-Antikörper wurde für WB, IHC, IF, IC und LCI validiert. Er ist geeignet, ChT in Proben von Maus zu detektieren.

Kurzübersicht für ChT Antikörper (Extracellular) (ABIN7043267)

Target: ChT (High Affinity Choline Transporter (ChT))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ChT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Produktdetails anti-ChT Antikörper

Bindungsspezifität: AA 299-311, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Choline Transporter (SLC5A7)

Spezifität: 4th extracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,human - 12,13 amino acid residues identical

Produktmerkmale: Anti-Choline Transporter (SLC5A7) (extracellular) Antibody is directed against an epitope of mouse CHT. Anti-Choline Transporter (SLC5A7) (extracellular) Antibody (ABIN7043267 and ABIN7044218) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize CHT from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DWNQTAYGYPDPK, corresponding to amino acid residues 299-311 of mouse Choline transporter

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu ChT

Target: ChT (High Affinity Choline Transporter (ChT))

Andere Bezeichnung: SLC5A7

Hintergrund: Na+/Cl--Dependent high affinity choline transporter 1, Hemicholinium-3-sensitive choline transporter, CHT, CHT1,In cholinergic neurons, acetylcholine is synthesized from choline and acetyl-coenzyme A (acetyl-CoA). Following depolarization, acetylcholine is released to the synaptic cleft via synaptic vesicles and activates muscarinic and nicotinic receptors located on postsynaptic membranes1,2. Acetylcholine is then rapidly hydrolyzed into choline and acetate. Since choline is not synthesized de novo and thereby only made available through diet, choline is recycled back into the cells in order to regenerate acetylcholine. For this purpose, choline is taken up by the high affinity choline transporter (CHT)1,2.CHT has thirteen transmembrane domains, an extracellular N-terminus and an intracellular C-terminus3,4. CHT belongs to the Na+-dependent glucose transporter family (SLC5). The activity of the transporter can be confirmed by its sensitivity to hemicholimium-3 (HC-3) which inhibits the transport of choline with a Ki of 10-100 nM1. The activity of CHT reflects that of neurons1,5 i.e. increased CHT activity indicates an increase in neuronal activity. The increase in the activity of the transporter is due to the increase in the number of transporters and not due to the increase in activity per se. In addition CHT activity is also regulated by second messengers. Phosphorylation of the transporter also seems to determine the activity as it has many consensus phosphorylation sites present in the C-terminal1,2.Expression studies reveal that CHT is expressed solely in cholinergic neurons making the transporter a useful marker for detecting these types of neurons6.

Alternative names: Choline Transporter (SLC5A7), Na+/Cl--Dependent high affinity choline transporter 1, Hemicholinium-3-sensitive choline transporter, CHT, CHT1

Gen-ID: 63993

NCBI Accession: NM_001305005

UniProt: Q8BGY9