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ChT Antikörper (Extracellular)

Der Rabbit Polyclonal Anti-ChT-Antikörper wurde für WB, IHC, IF, IC, LCI validiert. Er ist geeignet, ChT in Proben von Mouse zu detektieren.
Produktnummer ABIN7043267

Kurzübersicht für ChT Antikörper (Extracellular) (ABIN7043267)

Target

Alle ChT Antikörper anzeigen
ChT (High Affinity Choline Transporter (ChT))

Reaktivität

  • 11
  • 11
  • 10
  • 9
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Maus

Wirt

  • 11
  • 10
Kaninchen

Klonalität

  • 11
  • 10
Polyklonal

Konjugat

  • 15
  • 2
  • 2
  • 2
Dieser ChT Antikörper ist unkonjugiert

Applikation

  • 12
  • 9
  • 7
  • 5
  • 3
  • 3
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)
  • Bindungsspezifität

    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 299-311, Extracellular

    Verwendungszweck

    A Rabbit Polyclonal Antibody to Choline Transporter (SLC5A7)

    Spezifität

    4th extracellular loop

    Kreuzreaktivität

    Human, Maus, Ratte

    Homologie

    Rat,human - 12,13 amino acid residues identical

    Produktmerkmale

    Anti-Choline Transporter (SLC5A7) (extracellular) Antibody is directed against an epitope of mouse CHT. Anti-Choline Transporter (SLC5A7) (extracellular) Antibody (ABIN7043267 and ABIN7044218) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize CHT from rat, mouse and human samples.

    Aufreinigung

    Affinity purified on immobilized antigen.

    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)DWNQTAYGYPDPK, corresponding to amino acid residues 299-311 of mouse Choline transporter

    Isotyp

    IgG
  • Applikationshinweise

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

    Application Dilutions Western blot wb: 1:200

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    0.2 mL double distilled water (DDW).

    Konzentration

    1 mg/mL

    Buffer

    PBS pH 7.4

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target

    ChT (High Affinity Choline Transporter (ChT))

    Andere Bezeichnung

    SLC5A7

    Hintergrund

    Na+/Cl--Dependent high affinity choline transporter 1, Hemicholinium-3-sensitive choline transporter, CHT, CHT1,In cholinergic neurons, acetylcholine is synthesized from choline and acetyl-coenzyme A (acetyl-CoA). Following depolarization, acetylcholine is released to the synaptic cleft via synaptic vesicles and activates muscarinic and nicotinic receptors located on postsynaptic membranes1,2. Acetylcholine is then rapidly hydrolyzed into choline and acetate. Since choline is not synthesized de novo and thereby only made available through diet, choline is recycled back into the cells in order to regenerate acetylcholine. For this purpose, choline is taken up by the high affinity choline transporter (CHT)1,2.CHT has thirteen transmembrane domains, an extracellular N-terminus and an intracellular C-terminus3,4. CHT belongs to the Na+-dependent glucose transporter family (SLC5). The activity of the transporter can be confirmed by its sensitivity to hemicholimium-3 (HC-3) which inhibits the transport of choline with a Ki of 10-100 nM1. The activity of CHT reflects that of neurons1,5 i.e. increased CHT activity indicates an increase in neuronal activity. The increase in the activity of the transporter is due to the increase in the number of transporters and not due to the increase in activity per se. In addition CHT activity is also regulated by second messengers. Phosphorylation of the transporter also seems to determine the activity as it has many consensus phosphorylation sites present in the C-terminal1,2.Expression studies reveal that CHT is expressed solely in cholinergic neurons making the transporter a useful marker for detecting these types of neurons6.

    Alternative names: Choline Transporter (SLC5A7), Na+/Cl--Dependent high affinity choline transporter 1, Hemicholinium-3-sensitive choline transporter, CHT, CHT1

    Gen-ID

    63993

    NCBI Accession

    NM_001305005

    UniProt

    Q8BGY9
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