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FFAR2 Antikörper (Extracellular)

FFAR2 Reaktivität: Human WB, IF (cc), LCI Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN7043139
  • Target Alle FFAR2 Antikörper anzeigen
    FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))
    Bindungsspezifität
    • 16
    • 15
    • 12
    • 8
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 153-167, Extracellular
    Reaktivität
    • 57
    • 18
    • 18
    • 3
    • 2
    • 1
    • 1
    Human
    Wirt
    • 74
    • 1
    Kaninchen
    Klonalität
    • 74
    • 1
    Polyklonal
    Konjugat
    • 28
    • 7
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser FFAR2 Antikörper ist unkonjugiert
    Applikation
    • 51
    • 27
    • 26
    • 16
    • 15
    • 14
    • 13
    • 7
    • 6
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)
    Verwendungszweck
    A Rabbit Polyclonal Antibody to Human Free Fatty Acid Receptor 2 (GPR43)
    Spezifität
    2nd extracellular loop
    Kreuzreaktivität (Details)
    Not recommended for mouse or rat samples.
    Homologie
    Human only
    Produktmerkmale
    Anti-Human GPR43/FFAR2 (extracellular) Antibody (ABIN7043139, ABIN7044283 and ABIN7044284)) is a highly specific antibody directed against an epitope of the human Free fatty acid receptor 2. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize FFAR2 from human samples only. It will not recognize the protein from rat or mouse samples.
    Aufreinigung
    Affinity purified on immobilized antigen.
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)TEQVRSGNEIT(S)YEN, corresponding to amino acid residues 153-167 of human FFAR2

    Isotyp
    IgG
    Top Product
    Discover our top product FFAR2 Primärantikörper
  • Applikationshinweise

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

    Application Dilutions Western blot wb: 1:200

    Kommentare

    Negative Control: BLP-FR015

    Blocking Peptide: BLP-FR015

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    0.2 mL double distilled water (DDW).
    Konzentration
    1 mg/mL
    Buffer
    PBS pH 7.4
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))
    Andere Bezeichnung
    FFAR2 (FFAR2 Produkte)
    Hintergrund
    G-protein coupled receptor 43, Free fatty acid receptor 2,Free fatty acids (FFAs) are essential nutrients that are also involved in signal transduction through FFA receptors, members of the G-protein coupled receptor superfamily.FFAR2/GPR43 is an FFA receptor that is activated specifically by short fatty acids (acetate, propionate and butyrate), that are the products of bacterial fermentation in the gut1.GPR43 is expressed in adipose tissue, intestines, and immune tissues2 and its stimulation by interaction with short fatty acids inhibits cAMP production, activates the extracellular signal-regulated kinase (ERK) cascade via interactions with the Gi/o family of G-proteins. This increases intracellular Ca2+ levels, and promotes activation of the mitogen-activated protein kinase (MAPK) cascade through interactions with the Gq family of G-proteins1.These processes, mediated by GPR43 receptor regulate the host energy homeostasis and hence GPR43 is shown to be related to metabolic disorders, such as obesity and diabetes1.Because of its pivotal role in energy homeostasis and in regulating many cellular processes, GPR43 has many phenotypic effects: mutant mice are hyper susceptible to developing intestinal carcinogenesis3, Klebsiella pneumoniae infection4 and to increased differentiation of bone marrow cells into osteoclasts5. Studies have shown the role of GPR43 in regulating inflammatory responses, which can lead to colitis and asthma6,7. The various activities mediated by GPR43 makes it an appealing therapeutic target.

    Alternative names: GPR43/FFAR2, G-protein coupled receptor 43, Free fatty acid receptor 2
    Gen-ID
    2867
    NCBI Accession
    NM_005306
    UniProt
    O15552
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