CCR9 Antikörper (Extracellular)

Produktnummer ABIN7043036

Produktdetails anti-CCR9 Antikörper

Target: CCR9 (Chemokine (C-C Motif) Receptor 9 (CCR9))

Bindungsspezifität: AA 205-218, Extracellular

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCR9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Verwendungszweck: A Rabbit Polyclonal Antibody to CCR9 Chemokine Receptor

Spezifität: 2nd extracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,rat - 10,14 amino acid residues identical

Produktmerkmale: Anti-CCR9 (extracellular) Antibody (ABIN7043036, ABIN7044177 and ABIN7044178)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize CCR9 from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DESTKLKSAVLTLK, corresponding to amino acid residues 205-218 of human CCR9

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: BLP-CR029

Blocking Peptide: BLP-CR029

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu CCR9

Target: CCR9 (Chemokine (C-C Motif) Receptor 9 (CCR9))

Andere Bezeichnung: CCR9 (CCR9 Produkte)

Hintergrund: C-C chemokine receptor type 9, CC-CKR-9, G-protein coupled receptor 28, GPR28, GPR-9-6, CDw199,CCR9 (C-C chemokine receptor type 9) belongs to the β-chemokine receptor family of the G-protein coupled receptor superfamily. Chemokine receptors play a crucial role in cell migration and are considered to be important therapeutic targets for inflammatory diseases and in tumor chemoresistance and metastasis. In addition, high expression of CCR9 has been reported in a wide range of cancers including melanoma, ovarian cancer, lung cancer, breast cancer and colon cancer. Thus, CCR9 is considered to be a potential tumor biomarker in diagnosis and therapy of cancer1,2. Under physiological conditions, CCR9 is mainly expressed in immature T lymphocytes and on the surface of intestinal cells2.CCR9 receptor interacts with its specific ligand- chemokine ligand 25 (CCL25, thymus-expressed chemokine) in a two-step binding mechanism in which the C-terminal region of CCL25 first binds the N-terminus region and extracellular loops of the receptor followed by the binding of the N-terminus of the chemokine. The two-step biding mechanism stabilizes the receptor in an active conformation that facilitates intracellular signaling1.

Alternative names: CCR9, C-C chemokine receptor type 9, CC-CKR-9, G-protein coupled receptor 28, GPR28, GPR-9-6, CDw199

Gen-ID: 10803

NCBI Accession: NM_001256369

UniProt: P51686