CACNB1 Antikörper (Intracellular)

Produktnummer ABIN7042984

Produktdetails anti-CACNB1 Antikörper

Target: CACNB1 (Calcium Channel, Voltage-Dependent, beta 1 Subunit (CACNB1))

Bindungsspezifität: AA 456-471, Intracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CACNB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Verwendungszweck: A Rabbit Polyclonal Antibody to CACNB1 (CaVβ1) Subunit

Spezifität: Intracellular, adjacent to the C-terminus

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse - identical, human - 14,16

Produktmerkmale: Anti-CACNB1 Antibody is directed against an epitope of rat CaVβ1. Anti-CACNB1 Antibody (ABIN7042984, ABIN7044049 and ABIN7044050) can be used in western blot and immunohistochemistry applications. It has been designed to recognize CaVβ1 from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DRATGEHASVHEYPGE, corresponding to amino acid residues 456-471 of rat CACNB1 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:800

Kommentare:

Negative Control: (ABIN7234923)

Blocking Peptide: (ABIN7234923)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu CACNB1

Target: CACNB1 (Calcium Channel, Voltage-Dependent, beta 1 Subunit (CACNB1))

Andere Bezeichnung: CACNB1 (CACNB1 Produkte)

Hintergrund: CaVβ1, CAB1, CACNLB1, Voltage-dependent L-type calcium channel subunit beta-1,Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+ conducting pores in the plasma membrane1. Based on their electrophysiological and pharmacological properties, CaV channels have traditionally been classified into L, T, N, P, Q, and R types2. L-type Ca2+ channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca2+ flow across the membrane, and the subunits α2δ, γ, and β3. CaVβ subunits play critical roles in membrane trafficking of the channel complex and regulation of voltage-dependent gating. The CaVβ subunit binds to the endoplasmic reticulum (ER) retention signal in the I-II loop of the α1-subunit, which allows channels to traffic to the surface membrane4. Furthermore, CaVβ subunits not only allow for membrane trafficking of the channel complex, they also can play a role in determining the subcellular localization of channels on the surface membrane5. There are four distinct CaVβ subunits CaVβ1, CaVβ2, CaVβ3 and CaVβ46.Three splice variants exist for the β1 subunit: β1a, β1b and β1c. β1a is known to be expressed in skeletal muscle and brain, but not in smooth muscle or heart. β1a appears to be important for the functional expression of the α1 subunit in skeletal muscle7. β1b was identified by cloning in rat brain, heart and hippocampus, and differs from β1a by having a deletion of ~50 amino acids at residue 209, and having a 120-residue C-terminal elongation. β1c was cloned from human heart and hippocampus and has the same deletion as β1b, but lacks the C-terminal extension.

Alternative names: CACNB1, CaVbeta1, CAB1, CACNLB1, Voltage-dependent L-type calcium channel subunit beta-1

Gen-ID: 50688

NCBI Accession: NM_000723

UniProt: P54283