Calcitonin Receptor Antikörper (Extracellular)

Produktnummer ABIN7042978

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Calcitonin Receptor in WB, IF, IC und LCI. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für Calcitonin Receptor Antikörper (Extracellular) (ABIN7042978)

Target: Calcitonin Receptor (CALCR)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Calcitonin Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Produktdetails anti-Calcitonin Receptor Antikörper

Bindungsspezifität: AA 201-215, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Calcitonin Receptor

Spezifität: 1st extracellular loop

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse - identical, human - 14,15 amino acid residues identical

Produktmerkmale: Anti-Calcitonin Receptor (extracellular) Antibody is directed against an epitope of rat CT-R. Anti-Calcitonin Receptor Antibody (ABIN7042978, ABIN7044190 and ABIN7044191) can be used in western blot and live cell imaging applications. It has been designed to recognize CT-R from human, rat and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HLVEVVPNGDLVRRD, corresponding to amino acid residues 201-215 of rat CALCR

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7234900)

Blocking Peptide: (ABIN7234900)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Calcitonin Receptor

Target: Calcitonin Receptor (CALCR)

Andere Bezeichnung: CALCR

Hintergrund: CALCR, CT receptor, CT-R, C1A/C1B,the calcitonin receptor (CTR) is a class B G-protein coupled receptor (GPCR) that signals via activation of cAMP and protein kinase C (PKC). It is one of 15 human class B GPCRs1,2. Apart from CTR, calcitonin receptor-like receptor (CTLR) is the only other B-class GPCR to bind calcitonin. To date, there are three known human CTR mRNA isoforms arising from alternative splicing3.Calcitonin itself is a peptide hormone that contributes to bone calcification by direct inhibition of osteoclasts which resorb the bony tissue. It is part of a six-member family that has varied functionalities. By binding to receptor activity modifying proteins (RAMPs), CTR and CTLR can form receptors to every member of the calcitonin family. For instance, RAMP1 and CTR construct the calcitonin gene-related peptide (CGRP) receptor.Both CTR and CTLR have seven transmembrane domains, an intracellular C-terminal, and an extracellular N-terminal. To activate the receptors, their N-terminal must first capture the C-terminal of the ligand and then carried to the transmembrane loops where it bestows a conformational change upon them.Besides the bone, CTR has prominent roles in the kidney, CNS, placenta, ovaries, sperm, lung, and in the brain4,5.In osteoclasts, the expression of CTRs declines with age. Interestingly, polymorphism of the CTR gene is strongly associated with decreased fracture-risk in post-menopausal women3,6.

Alternative names: Calcitonin Receptor, CALCR, CT receptor, CT-R, C1A/C1B

Gen-ID: 116506

NCBI Accession: NM_001742

UniProt: P32214

Pathways: cAMP Metabolic Process