AQP9 Antikörper (Intracellular)

Produktnummer ABIN7042945

Produktdetails anti-AQP9 Antikörper

Target: AQP9 (Aquaporin 9 (AQP9))

Bindungsspezifität: AA 4-19, Intracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AQP9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Verwendungszweck: A Rabbit Polyclonal Antibody to Aquaporin 9 Channel

Spezifität: Intracellular, N-terminus

Kreuzreaktivität: Maus, Ratte

Kreuzreaktivität (Details): Unlikely to recognize human samples.

Homologie: Mouse - 13,16 amino acid residues identical

Produktmerkmale: Anti-Aquaporin 9 Antibody (ABIN7042945 and ABIN7045206)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize AQP9 from mouse and rat samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EKDGAKKSLMQRLALK, corresponding to amino acid residues 4-19 of rat AQP9

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Kommentare:

Cited Application: IHC

Negative Control: BLP-QP009

Blocking Peptide: BLP-QP009

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu AQP9

Target: AQP9 (Aquaporin 9 (AQP9))

Andere Bezeichnung: AQP9 (AQP9 Produkte)

Hintergrund: AQP9, Aquaglyceroporin-9,Aquaporin 9 (AQP-9) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes.1 The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are permeated primarily by water (AQP-1, AQP-2, AQP4, AQP-5, AQP-6, AQP-8), and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3, AQP-7, AQP-9 and AQP-10.The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit contains four pores.2AQP-9 is expressed in several tissues including the brain, liver, testis and spleen. In the liver AQP-9 has a central role in glycerol metabolism as demonstrated in experiments using AQP-9 knockout mice: mice deficient in AQP-9 expression showed enhanced plasma levels of glycerol and triglycerides.3

Alternative names: Aquaporin 9, AQP9, Aquaglyceroporin-9

Gen-ID: 65054

NCBI Accession: NM_020980

UniProt: P56627