Aquaporin 7 Antikörper (Intracellular)

Produktnummer ABIN7042943

Produktdetails anti-Aquaporin 7 Antikörper

Target: Aquaporin 7 (AQP7)

Bindungsspezifität: AA 7-24, Intracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Aquaporin 7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Verwendungszweck: A Rabbit Polyclonal Antibody to Aquaporin 7 Channel

Spezifität: Intracellular, N-terminus

Kreuzreaktivität: Maus, Ratte

Homologie: 18 amino acid residues identical,Mouse - 15, human - 11

Produktmerkmale: Anti-Aquaporin 7 Antibody (ABIN7042943, ABIN7045203 and ABIN7045204) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize from mouse and rat samples, and may recognize the channel from human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: ENIQSVLQKTWVREFLAE, corresponding to amino acid residues 7-24 of rat AQP7

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:500

Kommentare:

Cited Application: IHC

Negative Control: (ABIN7234775)

Blocking Peptide: (ABIN7234775)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Aquaporin 7

Target: Aquaporin 7 (AQP7)

Andere Bezeichnung: AQP7 (AQP7 Produkte)

Hintergrund: AQP7, Aquaglyceroporin-7, AQPap, Aquaporin adipose,Aquaporin 7 (AQP-7) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.The aquaporins can be divided into two functional groups based on their permeability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-7, AQP-3, AQP-9 and AQP-10.The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit contains four pores.AQP-7 is expressed in ovary, testis, kidney and adipose tissue.The function of AQP-7 in adipose tissue attracted much interest as mice deficient in AQP-7 developed adult-onset obesity and Type 2 Diabetes. AQP-7 modulates adipocyte glycerol permeability thereby controlling triglyceride accumulation and fat cell size.

Alternative names: Aquaporin 7, AQP7, Aquaglyceroporin-7, AQPap, Aquaporin adipose

Gen-ID: 29171

NCBI Accession: NM_001170

UniProt: P56403