ADORA1 Antikörper (AA 213-229)

Produktnummer ABIN7042882

Produktdetails anti-ADORA1 Antikörper

Target: ADORA1 (Adenosine A1 Receptor (ADORA1))

Bindungsspezifität: AA 213-229

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADORA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Verwendungszweck: A Rabbit Polyclonal Antibody to Adenosine A1 Receptor

Spezifität: 3rd intracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,rat,bovine,canis,pig - identical

Produktmerkmale: Anti-Adenosine A1 Receptor Antibody is directed against human adenosine A1 receptor. Anti-Adenosine A1 Receptor Antibody (ABIN7042882, ABIN7043894 and ABIN7043895)) can be used in western blot analysis, immunocytochemical and immunohistochemical applications. It has been designed to recognize adenosine A1R from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Güteklasse: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KKVSASSGDPQKYYGKE, corresponding to amino acid residues 213-229 of human A1AR

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: BLP-AR006

Blocking Peptide: BLP-AR006

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu ADORA1

Target: ADORA1 (Adenosine A1 Receptor (ADORA1))

Andere Bezeichnung: ADORA1 (ADORA1 Produkte)

Hintergrund: ADORA1, A1AR, Adenosine A1R,Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart.1,2 Adenosine exerts its actions via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop that is important for binding G proteins.1-3 The adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs.1-3A1AR is widely distributed and has been well characterized. High expression of A1AR is found in the brain (mainly in the cortex, cerebellum, and hippocampus), dorsal horn of the spinal cord, adrenal gland, and atria, and to a lower extent in several other tissues including adipose tissue, the colon, and kidney.2,4A1AR modulates the activity of several ion channels. Activation of A1AR (by adenosine, its major agonist) inhibits N-type Ca2+ channels via a voltage-dependent, pertussis toxin (PTX)-sensitive pathway in neurons of the rat major pelvic ganglia (MPG).5Since A1AR is the most prominent adenosine receptor in adipocytes, it has become a natural target for research on obesity, which is a major health problem.6,7 A possible role in cell proliferation and carcinogenesis has also been suggested for A1AR.8-10

Alternative names: Adenosine A1 Receptor, ADORA1, A1AR, Adenosine A1R

Gen-ID: 134

NCBI Accession: NM_000674

UniProt: P30542

Pathways: EGFR Signaling Pathway, Negative Regulation of Hormone Secretion, Synaptic Membrane