GLI Family Zinc Finger Protein 3 (AA 303-561) Antikörper

Produktnummer ABIN7439492

Produktdetails

Target: GLI Family Zinc Finger Protein 3

Bindungsspezifität: AA 303-561

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC)

Verwendungszweck: Polyclonal Antibody to GLI Family Zinc Finger Protein 3 (GLI3)

Spezifität: The antibody is a rabbit polyclonal antibody raised against GLI3. It has been selected for its ability to recognize GLI3 in immunohistochemical staining and western blotting.

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant GLI Family Zinc Finger Protein 3 (GLI3) corresdonding to Ser303~Glu561 (Accession # Q61602)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Antigendetails

Target: GLI Family Zinc Finger Protein 3

Hintergrund: GCPS, PHS, PAP-A, PAPA, PAPA1, PAPB, ACLS, PPDIV, Greig Cephalopolysyndactyly Syndrome, Glioma-Associated Oncogene Family Zinc Finger 3, GLI3 form of 190 kDa