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Rekombinanter EpCAM Antikörper (Extracellular Domain)

Name: Rekombinanter EpCAM Antikörper (Extracellular Domain)
Brand: antibodies-online
SKU: ABIN6939996
Price: 663.89 EUR
Availability: InStock

EPCAM Reaktivität: Human WB, IHC, StM Wirt: Kaninchen Monoclonal EGP40-2041R unconjugated Recombinant Antibody

Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/2041R). (Rekombinanter EpCAM Antikörper (Extracellular Domain))

IHC

Western Blot of MCF-7 cell lysate using EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/2041R). (Rekombinanter EpCAM Antikörper (Extracellular Domain))

Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/2041R). (Rekombinanter EpCAM Antikörper (Extracellular Domain))

IHC

SDS-PAGE Analysis Purified EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/2041R). (Rekombinanter EpCAM Antikörper (Extracellular Domain))

SDS

Western Blot of human lung lysate using EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/2041R). (Rekombinanter EpCAM Antikörper (Extracellular Domain))

5 Abbildungen

Produktnummer ABIN6939996

Datenblatt (PDF)

Target Alle EpCAM (EPCAM) Antikörper anzeigen

EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Antikörpertyp

Recombinant Antibody
Bindungsspezifität
56

42

30

27

16

16

14

12

12

11

10

8

5

4

4

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 100-224, Extracellular Domain
Reaktivität
302

87

37

2

1

1
Human
Wirt
164

146

20

1

1
Kaninchen
Passende Sekundärantikörper
Klonalität
211

116

3
Monoklonal
Konjugat
156

22

20

12

9

9

8

8

8

8

8

5

5

5

5

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
Dieser EpCAM Antikörper ist unkonjugiert
Applikation
214

195

124

117

87

33

30

26

24

21

17

16

15

11

4

3

3

2

2

2

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Spezifität

Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Immunogen

Recombinant human EpCAM fragment from extracellular domain (around aa100-224) (exact sequence is proprietary)

Klon

EGP40-2041R

Isotyp

IgG

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anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
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anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM Reaktivität: Human WB, FACS, IF, IHC, StM Wirt: Maus Monoclonal EGP40-1372 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 20-60), (N-Term) antibody
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anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 280-350), (Cytoplasmic Domain) antibody
EPCAM Reaktivität: Human, Maus, Ratte IHC, StM Wirt: Maus Monoclonal EGP40-1110 unconjugated

Applikationshinweise

Positive Control: MCF-7 or HT29 cells. Breast tumor.
Known Application: Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

200 μg/mL

Buffer

10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-80 °C

Informationen zur Lagerung

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit

24 months
Target

EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Andere Bezeichnung

TACSTD1 (EPCAM Produkte)

Molekulargewicht

40-43kDa

Gen-ID

4072

UniProt

P16422