SPAM1 Antikörper (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding))

Details for Product anti-SPAM1 Antibody No. ABIN6569119
Target
Reaktivität
Human, Maus, Ratte
26
2
2
Wirt
Kaninchen
25
1
Klonalität
Polyklonal
Konjugat
Dieser SPAM1 Antikörper ist unkonjugiert
3
2
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (IF), Western Blotting (WB)
17
13
3
1
1
1
1
Optionen
Immunogen Recombinant protein of human SPAM1
Isotyp IgG
Reinigung Affinity purification
Target
Andere Bezeichnung SPAM1 (SPAM1 Antibody Abstract)
Hintergrund

Synonyms: epididymis secretory sperm binding protein Li 96n,HEL-S-96n,HYA1,HYAL PH-20,HYAL PH20,Hyal-PH20,HYAL1,HYAL3,HYAL5,HYALP,Hyaluronidase PH-20,Hyaluronoglucosaminidase PH-20,MGC26532,PH-20 Hyaluronidase,PH20,PH20 Hyaluronidase,SPAG15,SPAM-1,Spam1,sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding),Sperm adhesion molecule 1,Sperm surface protein PH-20,Sperm surface protein PH20,zona pellucida binding

Background: Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1, another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht

Observed_MW: 47kDa

Calculated_MW: 57kDa/58kDa

Gen-ID 6677
UniProt P38567
Applikations-hinweise WB 1:500 - 1:2000 IF 1:50 - 1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1 mg/mL
Buffer Buffer: PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C. Avoid freeze / thaw cycles.
Bilder
Image no. 1 for anti-Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1) antibody (ABIN6569119) Western blot analysis of extracts of various cell lines, using SPAM1 antibody.
Image no. 2 for anti-Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1) antibody (ABIN6569119) Immunofluorescence analysis of HeLa cells using SPAM1 antibody.