Alkaline Phosphatase Antikörper (N-Term)

Produktnummer ABIN3029905

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Alkaline Phosphatase in WB. Er zeigt eine Reaktivität gegenüber Maus und Ratte.

Kurzübersicht für Alkaline Phosphatase Antikörper (N-Term) (ABIN3029905)

Target: Alkaline Phosphatase (ALP)

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Alkaline Phosphatase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Alkaline Phosphatase Antikörper

Bindungsspezifität: N-Term

Aufreinigung: Antigen affinity

Immunogen: An amino acid sequence from the N-terminus of rat ALPL (FVPEKEKDPSYWRQQ) was used as the immunogen for this tissue-nonspecific Alkaline Phosphatase antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting points. Titration of the Alkaline Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the Alkaline Phosphatase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu Alkaline Phosphatase

Target: Alkaline Phosphatase (ALP)

Andere Bezeichnung: Alkaline Phosphatase

Hintergrund: Alkaline phosphatase removes phosphate groups from the 5-prime end of DNA and RNA, and from proteins, at high pH . Most mammals have 4 different isozymes: placental, placental like, intestinal and non-tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts, the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine(PEA) and pyridoxal-5-prime-phosphate(PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

UniProt: P08289