TCR beta (Vbeta5.3-Related) Antikörper

Produktnummer ABIN2749039

Produktdetails

Target: TCR beta (Vbeta5.3-Related)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Functional Studies (Func)

Spezifität: The antibody MEM-262 recognizes an extracellular epitope on beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.

Kreuzreaktivität (Details): Other not tested, Human

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Endotoxin-Niveau: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Human thymoma cell line HPB-ALL.

Klon: MEM-262

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western blotting: This antibody uniquely recognizes denatured TCR beta chains under the conditions of Western blotting.
Functional application: This antibody activates T cells (V beta 5-related subset).
Flow cytometry: Recommended dilution: 1-4 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen

Pavlistová, Drbal, Hilgert, Horejsí: "A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor." in: Immunology letters, Vol. 88, Issue 2, pp. 105-8, (2003) (PubMed).

Antigendetails

Target: TCR beta (Vbeta5.3-Related)

Hintergrund: TCRVB5.3-related