ZBTB7A Antikörper (Zinc Finger and BTB Domain Containing 7A) (Cy5)

Details for Product anti-ZBTB7A Antibody No. ABIN1704343
Antigen
  • ZBTB7A
  • FBI-1
  • FBI1
  • LRF
  • ZBTB7
  • ZNF857A
  • pokemon
  • 9030619K07Rik
  • 9130006G12Rik
  • AI452336
  • Lrf
  • Pokemon
  • Zbtb7
  • OCZF
  • zinc finger and BTB domain containing 7A
  • zinc finger and BTB domain containing 7C
  • zinc finger and BTB domain containing 7a
  • ZBTB7A
  • ZBTB7C
  • Zbtb7a
Reaktivität
Human, Maus, Ratte (Rattus)
98
57
45
11
8
7
6
4
4
4
2
2
1
Wirt
Kaninchen
95
5
3
Klonalität
Polyklonal
Konjugat
Dieser ZBTB7A Antikörper ist konjugiert mit Cy5
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p))
80
37
34
23
13
6
6
2
1
Optionen
Immunogen KLH conjugated synthetic peptide derived from human Pokemon
Isotyp IgG
Reinigung Purified by Protein A.
Plasmids, Primers & others Plasmide, Primers & weitere ZBTB7A products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung Pokemon/ZBTB7 (ZBTB7A Antibody Abstract)
Hintergrund

Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy.

Synonyms: Factor binding IST protein 1, Factor that binds to inducer of short transcripts protein 1, FBI-1, FBI1, HIV-1 1st-binding protein 1, Leukemia/lymphoma related factor, LRF, Pokemon, TIP21, TTF-I interacting peptide 21, ZBTB7, ZBTB7A, Zinc finger and BTB domain-containing protein 7A, ZBT7A_HUMAN.

Gen-ID 51341
Applikations-hinweise IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store at 4°C
Haltbarkeit 12 months
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