Maus anti-Human Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin alpha and mu Polypeptides (IGJ) (Joining Chain) Antikörper

Details zu Produkt Nr. ABIN118394, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Joining Chain
3
3
2
2
1
Reaktivität
Human
13
3
3
Wirt
Maus
9
3
1
Klonalität (Klon)
Monoklonal ()
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen Human J Chain. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Klon MC19-9
Isotyp IgG1
Spezifität The antibody reacts with Human J chain as tested by ELISA using purified Human Ig fragments as the solid phase target antigen. The antibody does not detect J chain in intact IgM in ELISA assays due to steric hindrance. In Western blotting techniques SM1013P detects J chain in IgM only in reducing conditions.
Reinigung Affinity Chromatography on Protein A.
Andere Bezeichnung Immunoglobulin J Chain
Hintergrund Immunoglobulin J chain (IGJ) serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also helps to bind these immunoglobulins to secretory components.Synonyms: IGCJ, IGJ
Gen-ID 3512
NCBI Accession NP_653247
UniProt P01591
Applikationshinweise Immunohistochemistry on Frozen Sections (1/200). ELISA (20 μg/mL). Immunoprecipitation (20 μg/mL). Western blotting (only under Reducing Conditions).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1.0 mg/mL
Buffer TRIS buffered Saline containing 0.09 % Sodium Azide as preservative.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Allgemeine Veröffentlichungen Arnold, Wormald, Suter, Radcliffe, Harvey, Dwek, Rudd, Sim: "Human serum IgM glycosylation: identification of glycoforms that can bind to mannan-binding lectin." in: The Journal of biological chemistry, Vol. 280, Issue 32, pp. 29080-7, 2005 (PubMed).

Produkt verwendet in: Leithäuser, Bäuerle, Huynh, Möller: "Isotype-switched immunoglobulin genes with a high load of somatic hypermutation and lack of ongoing mutational activity are prevalent in mediastinal B-cell lymphoma." in: Blood, Vol. 98, Issue 9, pp. 2762-70, 2001 (PubMed).