anti-N-Ethylmaleimide-Sensitive Factor (NSF) Antikörper

Required for vesicle-mediated transport. Zusätzlich bieten wir Ihnen NSF Kits (10) und NSF Proteine (9) und viele weitere Produktgruppen zu diesem Protein an.

Alle Antikörper anzeigen Gen GeneID UniProt
NSF 4905 P46459
NSF 18195 P46460
NSF 60355 Q9QUL6
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Top anti-NSF Antikörper auf

Showing 10 out of 118 products:

Katalog Nr. Reaktivität Wirt Konjugat Applikation Bilder Menge Lieferzeit Preis Details
Rind (Kuh) Kaninchen Unkonjugiert IHC, WB WB Suggested Anti-NSF Antibody Titration:  0.2-1 ug/ml  Positive Control:  Human brain Sample Type: 1. NT-2 cells2.  mouse brain extractsPrimary Antibody Dilution: 2ug/mlSecondary Antibody Dilution: 1: 20,000Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceLane 1: 60ug/laneLane 2: 80ug/laneImage Submitted by: Yuzhi ChenUniversity of Arkansas for Medical Science 100 μL 2 bis 3 Tage
Human Kaninchen Unkonjugiert WB Western blot analysis of NSF expression in MCF7 (A), Raw264.7 (B), H9C2 (C) whole cell lysates. 200 μL 13 bis 14 Tage
Maus Kaninchen Unkonjugiert IP, IHC, WB   100 μg 2 bis 3 Tage
Human Kaninchen Unkonjugiert ELISA, IHC, WB Western blot analysis of NSF using anti-NSF antibody .  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each  Lane was loaded with 50ug of sample under reducing conditions.   Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: human HepG2 cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSF antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSF at approximately 82KD. The expected band size for NSF is at 82KD. IHC analysis of NSF using anti-NSF antibody . NSF was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-NSF Antibody  overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. 100 μg 4 bis 6 Tage
Human Kaninchen Unkonjugiert ICC, IHC, WB Figure. Western Blot; Sample: Lane1: Human 293T Cells; Lane2: Mouse Brain Tissue; Lane3: Mouse Testis Tissue. Figure. Western Blot; Sample: Recombinant NSF, Human. 100 μg 13 bis 16 Tage
Human Kaninchen Unkonjugiert ELISA, WB 100 μL Verfügbar
Human Kaninchen Unkonjugiert WB 0.1 mL 6 bis 8 Tage
Maus Kaninchen Unkonjugiert IHC, IHC (p), IP, WB Rat Brain: Formalin-Fixed, Paraffin-Embedded (FFPE) Anti-NSF antibody IHC of rat brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. 50 μg 11 bis 14 Tage
Human Kaninchen Unkonjugiert ELISA, ICC, IF, WB Western blot analysis of extracts from CoLo cells, using NSF antibody.The lane on the left is treated with the antigen-specific peptide. ABIN6275675 staining CoLo cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod 100 μL 11 bis 12 Tage
Human Kaninchen Unkonjugiert IHC, WB IHC on rat cerebellum (cryo sections) using Rabbit antibody to n-terminal of NSF (Vesicle fusing ATPase): whole serum (ABIN350527) at 1 : 2000 dilution. IHC on rat cerebellum (cryo sections) using Rabbit antibody to n-terminal of NSF (Vesicle fusing ATPase): whole serum (ABIN350527) at 1:2000 dilution. 100 μL 7 bis 8 Tage

Am meisten referenzierte anti-NSF Antikörper

  1. Human Polyclonal NSF Primary Antibody für ICC, IP - ABIN1742270 : Michaut, Tomes, De Blas, Yunes, Mayorga: Calcium-triggered acrosomal exocytosis in human spermatozoa requires the coordinated activation of Rab3A and N-ethylmaleimide-sensitive factor. in Proceedings of the National Academy of Sciences of the United States of America 2000 (PubMed)
    Show all 5 Pubmed References

  2. Human Polyclonal NSF Primary Antibody für WB - ABIN550144 : Matveeva, Whiteheart, Vanaman, Slevin: Phosphorylation of the N-ethylmaleimide-sensitive factor is associated with depolarization-dependent neurotransmitter release from synaptosomes. in The Journal of biological chemistry 2001 (PubMed)
    Show all 3 Pubmed References

  3. Rat (Rattus) Monoclonal NSF Primary Antibody für ICC, IP - ABIN1742269 : Ohno, Ishihara, Terada, Kikuta, Sofue, Kawai, Serikawa, Sasa: Preferential increase in the hippocampal synaptic vesicle protein 2A (SV2A) by pentylenetetrazole kindling. in Biochemical and biophysical research communications 2009 (PubMed)
    Show all 2 Pubmed References

Weitere Antikörper gegen NSF Interaktionspartner

Human N-Ethylmaleimide-Sensitive Factor (NSF) Interaktionspartner

  1. A large copy number variant (CNV) that encompasses part of the NSF gene contributes to cocaine dependence.

  2. NSF deficiency in HeLa cells barely affected cell viability, anterograde trafficking of vesicular stomatitis virus glycoprotein G and transferrin endocytosis.

  3. a processive helicase-like mechanism for NSF in which approximately 1 residue is unwound for every hydrolyzed ATP molecule.

  4. Single and multiple-marker analyses revealed a strong association between cocaine dependence and the NSF gene.

  5. Knockdown of TRX1 increases the level of S-nitrosylated NSF, prolongs the inhibition of exocytosis, and suppresses leukocyte

  6. NSF independent fusion of Salmonella-containing late phagosomes with early endosomes.

  7. analysis of human NSF possible binding mode to GABARAP and GATE-16

  8. binding of NSF to GluR2-containing AMPARs in stabilizing these receptors in the synaptic membrane and impeding their regulated endocytosis.

  9. role of interaction with GluR2 in regulation of AMPA receptors by brain-derived neurotrophic factor

  10. Nitric oxide regulates exocytosis by S-nitrosylation of NSF.

  11. Expressing a biochemically defined NSF mutant that lacks ATPase activity results in a dramatic inhibition of stimulated mast cell exocytosis.

  12. NSF dissociates the VAMP-2.SNAP-25.syntaxin 13 complex, but has no effect on the Hrs-containing complex.

  13. Pctaire1 phosphorylates N-ethylmaleimide-sensitive fusion protein

  14. dual regulation on hbeta2AR trafficking and signaling by NSF through direct binding to cargo receptor and its ATPase activity.

  15. Data show that NSF is an inclusion body component in neuronal intranuclear inclusion disease identified by anti-SUMO-1-immunocapture.

  16. Data show that phosphorylation of N-ethylmaleimide-sensitive factor on tyrosine residues prevents its SNARE complex dissociation activity and establish a role for PTP1B in the modulation of the membrane fusion machinery.

Mouse (Murine) N-Ethylmaleimide-Sensitive Factor (NSF) Interaktionspartner

  1. Munc13-1 and Munc18-1, but not CAPS-1/2, stabilize primed synaptic vesicles by preventing NSF-dependent de-priming.

  2. NSF may have a role in vascular instability in gram-positive lung infections

  3. that mutant LRRK2 may impair synaptic vesicle dynamics via aberrant phosphorylation of N-ethylmaleimide sensitive fusion

  4. identify conserved sequences in the 3'UTR of NSF as miR-33 responsive elements and show that Nsf is specifically recruited to the RNA-induced silencing complex following induction of miR-33

  5. Arr1 and NSF interaction are necessary for both maintenance and modulation of normal photoreceptor synaptic regulation and NSF-RP2 interaction may play an important role in membrane protein trafficking in the photoreceptor.

  6. These findings identify new roles for N-ethylmaleimide-sensitive factor and protein kinase C epsilon in regulating synaptic inhibition through downregulation of GABA(A) receptors

  7. interaction of AMPA receptors with N-ethylmaleimide-sensitive factor plays a critical role in incorporation of AMPA receptors into synapses and for their subsequent removal during cerebellar long term depression

  8. PICK1 and NSF dynamically regulate the synaptic delivery of GluR2-containing receptors during CARP and thus regulate the calcium permeability of AMPA receptors at excitatory synapses.

  9. H(2)O(2) regulates exocytosis by inhibiting N-ethylmaleimide sensitive factor (NSF), a protein that regulates membrane fusion events necessary for exocytosis.

  10. important role for NSF activity in regulating the cell surface stability of GABA(A) receptors.

  11. dual regulation on hbeta2AR trafficking and signaling by NSF through direct binding to cargo receptor and its ATPase activity.

  12. These results indicate that phosphorylation of GluR2 S880 and NSF and PICK1 binding to GluR2 dynamically regulate GluR2 recycling.

  13. Nitric oxide regulates exocytosis by S-nitrosylation of N-ethylmaleimide sensitive factor

Cow (Bovine) N-Ethylmaleimide-Sensitive Factor (NSF) Interaktionspartner

  1. Report depletion of the ATPase NSF from Golgi membranes with hypo-S-nitrosylation of vasorelevant proteins in endothelial cells exposed to monocrotaline pyrrole.

Xenopus laevis N-Ethylmaleimide-Sensitive Factor (NSF) Interaktionspartner

  1. NSF- and SNARE-mediated membrane fusion events are essential steps during Nuclear envelope formation downstream of Nup107 recruitment, and upstream of membrane flattening and completion of nuclear pore complex assembly.

Zebrafish N-Ethylmaleimide-Sensitive Factor (NSF) Interaktionspartner

  1. Molecular analysis of ndrg4 mutants shows that expression of snap25 and nsf are sharply decreased, revealing a role of ndrg4 in controlling vesicle exocytosis. This uncovers a previously unknown function of ndrg4 in regulating vesicle docking and nodes of Ranvier organization, at least through its ability to finely tune the expression of the t-SNARE/NSF machinery.

  2. results establish a role for Nsf in the maintenance of synaptic contacts between hair cells and afferent neurons, mediated in part via the secretion of trophic signaling factors

  3. Nsf is essential in the formation of the nodes of Ranvier in the vertebrate nervous system.

  4. Activation of PKCgamma is necessary for the activity-dependent trafficking of AMPARs in embryonic zebrafish and is NMDA and SNARE-dependent and requires AMPARs to associate with both NSF and PICK1.

  5. This study analyses of nsf mutant zebrafish reveal an unexpected role for NSF in hypothalamic development, with mutant 5 days post-fertilization larvae exhibiting a stage-dependent loss of neuroendocrine transcripts.

Arabidopsis thaliana N-Ethylmaleimide-Sensitive Factor (NSF) Interaktionspartner

  1. The mutant phenotype to the missense mutation D374N in the NSF gene, encoding the N-ethylmaleimide-sensitive factor, was linked a key component of membrane fusion.

NSF Antigen-Profil

Protein Überblick

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).

Genbezeichner und Symbole assoziert mit NSF

  • N-ethylmaleimide sensitive factor, vesicle fusing ATPase (NSF) Antikörper
  • N-ethylmaleimide sensitive fusion protein (Nsf) Antikörper
  • N-ethylmaleimide sensitive factor, vesicle fusing ATPase (Nsf) Antikörper
  • N-ethylmaleimide-sensitive factor (NSF) Antikörper
  • N-ethylmaleimide sensitive factor (nsf) Antikörper
  • N-ethylmaleimide-sensitive factor (BBOV_II007200) Antikörper
  • N-ethylmaleimide-sensitive fusion protein, putative (TGME49_318510) Antikörper
  • N-ethylmaleimide sensitive factor L homeolog (nsf.L) Antikörper
  • vesicle-fusing ATPase (LOC100180685) Antikörper
  • vesicle-fusing ATPase (LOC9310886) Antikörper
  • N-ethylmaleimide-sensitive factor a (nsfa) Antikörper
  • AAA-type ATPase family protein (NSF) Antikörper
  • 18.m06598 Antikörper
  • AI316878 Antikörper
  • AU020090 Antikörper
  • AU067812 Antikörper
  • N-ethylmaleimide sensitive factor Antikörper
  • NEM-sensitive fusion protein Antikörper
  • nsf Antikörper
  • si:bz18k17.1 Antikörper
  • SKD2 Antikörper
  • T1J1.4 Antikörper
  • T1J1_4 Antikörper
  • wu:fj33g11 Antikörper

Bezeichner auf Proteinebene für NSF

N-ethylmaleimide-sensitive factor-like protein , N-ethylmaleimide-sensitive fusion protein , NEM-sensitive fusion protein , vesicle-fusing ATPase , vesicular-fusion protein NSF , N-ethylmaleimide sensitive factor , suppressor of K(+) transport growth defect 2 , N-ethylmaleimide sensitive fusion protein , N-ethylmaleimide-sensitive factor , vesicle-fusing ATPase-like , Vesicle-fusing ATPase , Vesicular-fusion protein NSF

4905 Homo sapiens
18195 Mus musculus
60355 Rattus norvegicus
373397 Strongylocentrotus purpuratus
419972 Gallus gallus
454819 Pan troglodytes
490921 Canis lupus familiaris
504457 Bos taurus
780249 Xenopus (Silurana) tropicalis
5478472 Babesia bovis T2Bo
7901210 Toxoplasma gondii ME49
100024726 Monodelphis domestica
100049722 Xenopus laevis
100063690 Equus caballus
100174078 Pongo abelii
100180685 Ciona intestinalis
100472215 Ailuropoda melanoleuca
779070 Xenopus laevis
9310886 Arabidopsis lyrata subsp. lyrata
368483 Danio rerio
100624659 Sus scrofa
825830 Arabidopsis thaliana
101120344 Ovis aries
100734887 Cavia porcellus
100770898 Cricetulus griseus
715297 Macaca mulatta
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