anti-Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 2, 64kDa (CSTF2) Antikörper

CSTF2 encodes a nuclear protein with an RRM (RNA recognition motif) domain. Zusätzlich bieten wir Ihnen CSTF2 Proteine (4) und viele weitere Produktgruppen zu diesem Protein an.

Alle Antikörper anzeigen Gen GeneID UniProt
CSTF2 108062 Q8BIQ5
CSTF2 683927  
CSTF2 1478 P33240
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Top anti-CSTF2 Antikörper auf antikoerper-online.de

Showing 10 out of 82 products:

Katalog Nr. Reaktivität Wirt Konjugat Applikation Bilder Menge Anbieter Lieferzeit Preis Details
Rind (Kuh) Kaninchen Unkonjugiert IHC, WB WB Suggested Anti-CSTF2 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: 293T cell lysate CSTF2 is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit Anti-CSTF2 antibody Paraffin Embedded Tissue: Human Lung cell Cellular Data: alveolar cell Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X 100 μL Anmelden zum Anzeigen 2 bis 3 Tage
$289.00
Details
Rind (Kuh) Kaninchen Unkonjugiert WB WB Suggested Anti-Cstf2 Antibody Titration: 1.0 ug/ml Positive Control: Mouse Pancreas Rabbit Anti-Cstf2 antibody Formalin Fixed Paraffin Embedded Tissue: Human Testis Primary antibody Concentration: 1:100 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20x Exposure Time: 0.5-2.0sec 100 μL Anmelden zum Anzeigen 2 bis 3 Tage
$289.00
Details
Maus Ziege Unkonjugiert IP, ELISA HeLa lysate either in RIPA buffer (lanes 1, 2, 4, 6) or TX100 buffer (lanes 3, 5, 7) overexpressing Mouse CSTF2 (64 kDa) (DYKDDDDK tagged) immunoprecipitated using ABIN1686783 (2 µg) with Protein G-coated magnetic beads in lanes 6 and 7 and probed with anti-DYKDDDDK (1/1000). Mock-transfected HeLa in Lane 1. A 1/40th part of the lysate was loaded in lanes 2 and 3 before IP and in lanes 4 an 5 after IP. Primary incubations were for 1 hour. Detected by chemiluminescence. Data obtained from Dr P Grozdanov, Texas Tech University Health Sciences Center, USA. ABIN1686783 (2 µg) used to pull down CSTF2 (64 kDa) from Mouse Brain (lane 5) lysate (500 µg protein) while it cannot pull down CSTF2 (64 kDa) from Mouse Liver (lane 6) lysate (2 µg) protein) using Protein G-coated magnetic beads. A 1/40th part of the lysates were loaded in lanes 1 (Brain) and 2 (Liver) before IP and in lanes 3 (Brain) and 4 (Liver) after IP. Western blot probed with mouse anti-CSTF2 (64 kDa)/CSTF2 (64 kDa) antibody and detected by chemiluminescence. Data obtained from Dr P Grozdanov, Texas Tech University Health Sciences Center, USA. 100 μg Anmelden zum Anzeigen 6 bis 7 Tage
$429.84
Details
Rind (Kuh) Kaninchen Unkonjugiert IC, IF, IP, IHC, WB Immunofluorescent analysis of CstF-64 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Immunohistochemical analysis of CstF-64 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 200 μL Anmelden zum Anzeigen 13 bis 14 Tage
$487.50
Details
Human Kaninchen Unkonjugiert IF, ELISA, WB Western blot analysis of extracts from 293/K562/Jurkat cells, using CSTF2 Antibody. The lane on the right is treated with the synthesized peptide. Immunofluorescence analysis of HeLa cells, using CSTF2 Antibody. The picture on the right is treated with the synthesized peptide. 100 μg Anmelden zum Anzeigen 2 bis 3 Tage
$302.50
Details
Human Kaninchen Unkonjugiert EIA, WB 0.4 mL Anmelden zum Anzeigen 6 bis 8 Tage
$484.00
Details
Human Kaninchen Unkonjugiert WB Western blot analysis of anti-CSTF2 Antibody (N-term) (ABIN388655) in NCI-H460 cell line lysates (35 µg/lane). CSTF2(arrow) was detected using the purified polyclonal antibody. 400 μL Anmelden zum Anzeigen 10 bis 11 Tage
$385.00
Details
Human Kaninchen Unkonjugiert ELISA, IF, IHC, WB 100 μL Anmelden zum Anzeigen 16 Days
$181.73
Details
Human Kaninchen Unkonjugiert IHC, IHC (p), WB Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested. 50 μg Anmelden zum Anzeigen 11 bis 14 Tage
$727.83
Details
Human Kaninchen Unkonjugiert ELISA, ICC, IF, WB Western blot analysis of extracts from rat brain, using CSTF2 Antibody. ABIN6274236 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibody. 100 μL Anmelden zum Anzeigen 11 bis 12 Tage
$390.77
Details

Weitere Antikörper gegen CSTF2 Interaktionspartner

Mouse (Murine) Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 2, 64kDa (CSTF2) Interaktionspartner

  1. The results suggest that CstF-64 plays a key role in modulating the cell cycle in embryonic stem cells while simultaneously controlling histone mRNA 3' end processing.

  2. ELL2 and CstF-64 tracked together with RNA polymerase II across the Igh mu- and gamma-gene segments

Human Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 2, 64kDa (CSTF2) Interaktionspartner

  1. hnRNP H binds to two specific G-runs in exon 5a of ACHE and activates the distal alternative 3 splice site (ss) between exons 5a and 5b. Furthermore, hnRNP H competes for binding of CstF64 to the overlapping binding sites in exon 5a, and suppresses the selection of a cryptic polyadenylation site, which additionally ensures transcription of the distal 3 ss required for the generation of AChET isoform.

  2. CstF-64 is dispensable for the expression/3'-end processing of Star-PAP target mRNAs.CstF-64 and 3'-UTR cis-element determine Star-PAP specificity for target mRNA selection by excluding poly A polymerase.

  3. CstF64, an essential polyadenylation factor, is a master regulator of 3'-UTR shortening across multiple tumour types.

  4. CstF64 is central to the function of a heat-labile factor, composed of cleavage/polyadenylation specificity factor, symplekin, and cleavage stimulation factor 64 and appears to be at least partly responsible for its cell cycle regulation.

  5. CstF64 and CstF64tau modulate one another's expression and play overlapping as well as distinct roles in regulating global alternative polyadenylation profiles.

  6. CstF64 binds to thousands of dormant intronic PASs that are suppressed, at least in part, by U1 small nuclear ribonucleoproteins

  7. CSTF2 is likely to play an important role in lung carcinogenesis and be a prognostic biomarker in the clinic.

  8. nuclear accumulation of CstF-64 depends on binding to CstF-77 not symplekin; interaction between CstF-64/CstF-64Tau and CstF-77 are important for maintenance of nuclear levels of CstF complex components and intracellular localization, stability, function

  9. The Hinge domain is necessary for CstF-64 interaction with CstF-77 and consequent nuclear localization.

  10. dynamics of the CstF-64 RNA-binding domain, both free and bound to two GU-rich RNA sequences that represent polyadenylation regulatory elements, by NMR Spectroscopy

  11. The inactivity of the RSV poly(A) site was at least in part due to poor CstF binding since tethering CstF to the RSV substrate activated polyadenylation.

  12. CstF64 (cstf2) and not other CstF subunits induced by lipopolysaccharide (LPS) in murine macrophages and changes polyA site use

  13. Describes the induction of CSTF2 in the growth phase of cells.

CSTF2 Antigen-Profil

Protein Überblick

This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.

Genbezeichner und Symbole assoziert mit CSTF2

  • cleavage stimulation factor subunit 2 (CSTF2) Antikörper
  • cleavage stimulation factor, 3' pre-RNA, subunit 2 (cstf2) Antikörper
  • cleavage stimulation factor, 3' pre-RNA subunit 2 (Cstf2) Antikörper
  • cleavage stimulation factor subunit 2 (Cstf2) Antikörper
  • cleavage stimulation factor, 3' pre-RNA, subunit 2 (cstf2.L) Antikörper
  • 64kDa Antikörper
  • C630034J23Rik Antikörper
  • CSFT64 Antikörper
  • cstf-64 Antikörper
  • CSTF2 Antikörper
  • Cstf64 Antikörper
  • fb11e07 Antikörper
  • wu:fb11e07 Antikörper
  • zgc:56346 Antikörper
  • zgc:77730 Antikörper

Bezeichner auf Proteinebene für CSTF2

cleavage stimulation factor subunit 2 , cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa , cleavage stimulation factor 64 kilodalton subunit , CF-1 64 kDa subunit , CSTF 64 kDa subunit , Cleavage stimulation factor 64 kDa subunit , cstF-64 , alphaCstF-64 variant 4 , betaCstF-64 variant 1 , betaCstF-64 variant 3 , cleavage stimulation factor 64 kDa subunit , cleavage stimulation factor, 3' pre-RNA subunit 2, 64 kDa , betaCstF-64 variant 2

GENE ID SPEZIES
422258 Gallus gallus
473699 Pan troglodytes
100057347 Equus caballus
100170457 Xenopus (Silurana) tropicalis
386806 Danio rerio
100171993 Pongo abelii
108062 Mus musculus
683927 Rattus norvegicus
1478 Homo sapiens
492015 Canis lupus familiaris
282588 Bos taurus
379871 Xenopus laevis
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