anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) Antikörper

CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. Zusätzlich bieten wir Ihnen CDC20 Proteine (7) und und viele weitere Produktgruppen zu diesem Protein an.

Alle Antikörper anzeigen Gen GeneID UniProt
CDC20 991 Q12834
CDC20 107995 Q9JJ66
CDC20 64515 Q62623
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Top anti-CDC20 Antikörper auf antikoerper-online.de

Showing 10 out of 256 products:

Katalog Nr. Reaktivität Wirt Konjugat Applikation Bilder Menge Anbieter Lieferzeit Preis Details
Human Kaninchen Unkonjugiert IHC (p), WB Western blot analysis of Cdc20 using anti-Cdc20 antibody . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each  Lane was loaded with 50ug of sample under reducing conditions.  Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc20 antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc20 at approximately 55KD. The expected band size for Cdc20 is at 55KD. IHC analysis of Cdc20 using anti-Cdc20 antibody .Cdc20 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Cdc20 Antibody  overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. 100 μg Anmelden zum Anzeigen 4 bis 6 Tage
$280.00
Details
Rind (Kuh) Kaninchen Unkonjugiert IHC, WB WB Suggested Anti-CDC20 Antibody Titration:  0.2-1 ug/ml  ELISA Titer:  1:62500  Positive Control:  Hela cell lysate CDC20 is supported by BioGPS gene expression data to be expressed in HeLa 100 μL Anmelden zum Anzeigen 2 bis 3 Tage
$289.00
Details
Human Kaninchen Unkonjugiert EIA, IP, WB Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a, amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked headto- tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below. 0.1 mL Anmelden zum Anzeigen 6 bis 8 Tage
$594.00
Details
Human Kaninchen Unkonjugiert ICC, IF, IHC, IHC (p), IP Western Blot: CDC20 Antibody [ABIN252980] - Detection of Human CDC20 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDC20 antibody ABIN252980 used for IP at 3 mcg/mg lysate. CDC20 was also immunoprecipitated by rabbit anti- CDC20 antibody ABIN252981, which recognizes a downstream epitope. Immunohistochemistry-Paraffin: Cdc20 Antibody [ABIN2529807] - Detection of Mouse CDC20 by Immunohistochemistry. FFPE section of mouse teratoma. Affinity purified rabbit anti-CDC20 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit. 100 μL Anmelden zum Anzeigen 7 bis 9 Tage
$559.35
Details
Human Kaninchen Unkonjugiert ICC, IF, IHC, IHC (p), IP, PLA, WB Western Blot and Immunoprecipitation: CDC20 Antibody [ABIN252981] - Detection of Human CDC20 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB, 1 mg for IP, 20% of IP loaded) and 293T (T, 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CDC20 antibody ABIN252981 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CDC20 was also immunoprecipitated by rabbit anti-CDC20 antibody ABIN252980, which recognizes an upstream epitope. Immunohistochemistry-Paraffin: Cdc20 Antibody [ABIN2529818] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:250. 0.1 mL Anmelden zum Anzeigen 7 bis 9 Tage
$559.35
Details
Human Kaninchen Unkonjugiert WB Western blot analysis of CDC20 expression in Raji (A), HEK293T (B), HL60 (C) whole cell lysates. 200 μL Anmelden zum Anzeigen 13 bis 14 Tage
$487.50
Details
Human Kaninchen Unkonjugiert IF, IHC, WB Western blot analysis of extracts of various cell lines, using CDC20 antibody. 100 μL Anmelden zum Anzeigen 11 bis 13 Tage
$366.77
Details
Human Kaninchen Unkonjugiert ELISA, WB Western blot analysis of extracts from Jurkat/HeLa cells, using p55CDC Antibody. The lane on the right is treated with the synthesized peptide. 100 μg Anmelden zum Anzeigen 2 bis 3 Tage
$302.50
Details
Human Kaninchen Unkonjugiert ELISA, IHC, WB Western Blot (WB) analysis of specific cells using Cdc20 Polyclonal Antibody. 100 μL Anmelden zum Anzeigen Verfügbar
$181.73
Details
Fledermaus Kaninchen Unkonjugiert IHC, IHC (p), WB Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Anti-CDC20 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other for ... 50 μg Anmelden zum Anzeigen 11 bis 14 Tage
$727.83
Details

Am meisten referenzierte anti-CDC20 Antikörper

  1. Human Polyclonal CDC20 Primary Antibody für ICC, IF - ABIN252981 : Di Fiore, Pines: How cyclin A destruction escapes the spindle assembly checkpoint. in The Journal of cell biology 2010 (PubMed)
    Show all 3 Pubmed References

  2. Human Polyclonal CDC20 Primary Antibody für ICC, IF - ABIN252980 : Ahlskog, Björk, Elsing, Aspelin, Kallio, Roos-Mattjus, Sistonen: Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress. in Molecular and cellular biology 2010 (PubMed)
    Show all 2 Pubmed References

  3. Human Polyclonal CDC20 Primary Antibody für IP, WB - ABIN233786 : Yamamuro, Kano, Naito: Functions of FZR1 and CDC20, activators of the anaphase-promoting complex, during meiotic maturation of swine oocytes. in Biology of reproduction 2008 (PubMed)

  4. Xenopus laevis Monoclonal CDC20 Primary Antibody für CyTOF, ELISA - ABIN251088 : Sackton, Dimova, Zeng, Tian, Zhang, Sackton, Meaders, Pfaff, Sigoillot, Yu, Luo, King: Synergistic blockade of mitotic exit by two chemical inhibitors of the APC/C. in Nature 2014 (PubMed)

  5. Mouse (Murine) Polyclonal CDC20 Primary Antibody für IHC, WB - ABIN3021793 : Chen, Xu, Wang, Zhang, Wang, Xin, Liu, Jiang, Zhang, Zhang: CDK4 protein is degraded by anaphase-promoting complex/cyclosome in mitosis and reaccumulates in early G1 phase to initiate a new cell cycle in HeLa cells. in The Journal of biological chemistry 2017 (PubMed)

Weitere Antikörper gegen CDC20 Interaktionspartner

Human Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) Interaktionspartner

  1. we demonstrate that the CDC20-MAD2 complex could also be formed independently of the SAC. Moreover, in prolonged arrest caused by nocodazole treatment, the overall levels of the CDC20-MAD2 complex are gradually, but significantly, reduced and this is associated with lower levels of cyclin B1, which brings a new insight into the mechanism of mitotic "slippage" of the arrested cells.

  2. Cdc20 may be a promising molecular target for chemotherapy.

  3. Long non-coding RNA SPRY4-IT1 promotes cell proliferation and invasion by regulation of Cdc20 in pancreatic cancer cells

  4. Cdc20 functions as an important negative regulator of SMAR1 in higher grades of cancer

  5. Data provide support for the recent structure-based models and functionally dissect three elements of Cdc20 inhibition: sequestration of Cdc20 in the core mitotic checkpoint complex, sufficient at low Cdc20 concentrations; inhibition of a second Cdc20 through the Mad3 C terminus, independent of Mad2 binding to this Cdc20 molecule; and occupancy of the APC/C with full MCC, where Mad3 and Apc15 are involved.

  6. CDC20 gene, related to cell proliferation in protein complex A, might play momentous roles in the initiation and development of consecutive Trauma-Induced Sepsis.

  7. A mitotic phosphorylation site on Cdc20, known to be a substrate of PP2A(B56), modulates APC/C(Cdc20) assembly.

  8. c-Myc is a driver when combined with kRas/Akt3 oncogenic signals in gliomagenesis, whereas Cdc20 overexpression is a passenger

  9. The ABBA-KEN-ABBA amino acid motif cassette holds the Mitotic Checkpoint Complex (MCC) onto the Anaphase-Promoting Complex-Cyclosome (APC/C) by binding the two Cdc20 molecules in the MCC-APC/C complex.

  10. It discuses the roles of Cdc20 in SAC signalling and mitotic exit, describe how the integration of traditional approaches with emerging technologies has revealed new details of Cdc20 functions, comment about the potential of Cdc20 as a therapeutic target for the treatment of human malignancies.

  11. Prostate cancer-derived SPOP mutants failed to interact with Cdc20 to promote its degradation. As a result, SPOP-deficient prostate cancer cells with elevated Cdc20 expression became resistant to a pharmacological Cdc20 inhibitor.

  12. High CDC20 expression is associated with metastatic castration-resistant prostate cancer growth and reduces chemosensitivity .

  13. These results provide novel insight into the mechanisms underlying the aberrant capability of NUP98 oncoproteins to interact with APC/C(Cdc20) and to interfere with its function.

  14. In lung adenocarcinoma patients, overexpression of cell division cycle 20 was significantly associated with bigger primary tumor size, higher MKI67 level, higher DNA ploidy level, and poor prognosis.

  15. CDC20 may have a role in carcinoma of the breast, colon, endometrium, and prostate

  16. Overexpression of Cdc20 may serve as an independent predictor for biochemical recurrence in patients of clinically localized prostate cancer undergoing laparoscopic radical prostatectomy without neoadjuvant therapy.

  17. Results show that CYP1B1 may promote renal cell carcinoma development by inducing CDC20 expression and inhibiting apoptosis through the down-regulation of DAPK1.

  18. Study describes a positive feedback loop centred on cyclin A2-Cdk2 inhibition of interphase APC/C-Cdc20 to allow further cyclin A2 accumulation and mitotic entry.

  19. Bub1-Plk1-mediated phosphorylation of Cdc20 constitutes an anaphase-promoting complex or cyclosome-inhibitory mechanism that is parallel, but not redundant, to mitotic checkpoint complex formation.

  20. The presence of this segment correlates with SAC activity and efficient binding of CDC20 but not of MAD1 to kinetochores.

Xenopus laevis Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) Interaktionspartner

  1. Cdc20 auto-ubiquitylation does not play a major role in terminating Cdc20 activation.

  2. Dephosphorylation of Cdc20 is required for its loading and activation of the APC/C ubiquitin ligase.

  3. A role for the fizzy/cdc20 family of proteins in activation of the APC/C distinct from substrate recruitment is reported.

Mouse (Murine) Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) Interaktionspartner

  1. Cdc20 hypomorphism causes chromatin bridging and chromosome misalignment, revealing a requirement for Cdc20 in efficient sister chromosome separation and chromosome-microtubule attachment.

  2. The physiologically effective threshold level of Cdc20 is high for female meiosis I.

  3. Results indicate that Cdc20 also contributes to post-anaphase activation of the APC/C.

  4. This cell division cycle protein is a key regulator of the cell cycle.

  5. The seven tandem WD motifs of Cdc20 they are required for speriolin binding and for localization of Cdc20 to the centrosomes and nucleus, suggesting that speriolin might regulate or stabilize the folding of Cdc20 during meiosis in spermatogenic cells

  6. Data suggest that Mad2 and BubR1 must cooperate to inhibit Cdc20 activity.

  7. Cdc20 is degraded through two independent degradation signals (degrons), the KEN box and a newly described CRY box.

  8. Cdc20 and securin double mutant embryos could not maintain the metaphase arrest, suggesting a role of securin in preventing mitotic exit

  9. Expression of the cdc20 gene is down-regulated by zif268 in neuronal cells; altered expression of proteasome-regulatory genes following zif268 induction may be a key component of long-lasting CNS plasticity.

  10. Cdc20 is required for the anaphase onset of the first meiosis but not the second meiosis in mouse oocytes

  11. findings suggest a novel function of HSF1 frequently overexpressed in cancer cells, to inhibit APC/C activity by interacting with Cdc20, and to result in aneuploidy development and genomic instability

  12. Data show that the BubR1 N terminus binds to Cdc20 to inhibit APC/C activity in interphase, thereby allowing accumulation of cyclin B in G(2) phase prior to mitosis onset.

  13. Results indicate that the spindle assembly checkpoint-mediated inhibition of Cdc20 is an important tumor-suppressing mechanism.

Pig (Porcine) Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) Interaktionspartner

  1. porcine FZR1 and CDC20 work on the maintenance of meiotic arrest at the first meiotic prophase and on the exit from M1

CDC20 Antigen-Profil

Protein Überblick

CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation.

Genbezeichner und Symbole assoziert mit CDC20

  • cell division cycle 20 (cdc20) Antikörper
  • cell division cycle 20 (CDC20) Antikörper
  • cell division cycle 20 L homeolog (cdc20.L) Antikörper
  • cell division cycle 20 (Cdc20) Antikörper
  • cell division cycle 20 homolog (S. cerevisiae) (CDC20) Antikörper
  • 2310042N09Rik Antikörper
  • bA276H19.3 Antikörper
  • C87100 Antikörper
  • CDC20A Antikörper
  • fizzy1 Antikörper
  • p55CDC Antikörper
  • X-FZY Antikörper

Bezeichner auf Proteinebene für CDC20

cell division cycle 20 homolog , CDC20 cell division cycle 20 homolog , cell division cycle protein 20 homolog , fizzy1 , mmCdc20 , cell cycle protein p55CDC , p55CDC

GENE ID SPEZIES
394542 Xenopus (Silurana) tropicalis
991 Homo sapiens
398044 Xenopus laevis
107995 Mus musculus
64515 Rattus norvegicus
424563 Gallus gallus
482534 Canis lupus familiaris
397379 Sus scrofa
515376 Bos taurus
Ausgewählte Anbieter für anti-CDC20 (CDC20) Antikörper
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