PAPSS1 Protein (AA 24-624) (His tag)
Kurzübersicht für PAPSS1 Protein (AA 24-624) (His tag) (ABIN668017)
Target
Alle PAPSS1 Proteine anzeigenProtein-Typ
Spezies
Quelle
Applikation
Reinheit
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Proteineigenschaft
- AA 24-624
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Aufreinigungstag / Konjugat
- Dieses PAPSS1 Protein ist gelabelt mit His tag.
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Sequenz
- RATNV TYQAHHVSRN KRGQVVGTRG GFRGCTVWLT GLSGAGKTTV SMALEEYLVC HGIPCYTLDG DNIRQGLNKN LGFSPEDREE NVRRIAEVAK LFADAGLVCI TSFISPYTQD RNNARQIHEG ASLPFFEVFV DAPLHVCEQR DVKGLYKKAR AGEIKGFTGI DSEYEKPEAP ELVLKTDSCD VNDCVQQVVE LLQERDIVPV DASYEVKELY VPENKLHLAK TDAETLPALK INKVDMQWVQ VLAEGWATPL NGFMREREYL QCLHFDCLLD GGVINLSVPI VLTATHEDKE RLDGCTAFAL MYEGRRVAIL RNPEFFEHRK EERCARQWGT TCKNHPYIKM VMEQGDWLIG GDLQVLDRVY WNDGLDQYRL TPTELKQKFK DMNADAVFAF QLRNPVHNGH ALLMQDTHKQ LLERGYRRPV LLLHPLGGWT KDDDVPLMWR MKQHAAVLEE GVLNPETTVV AIFPSPMMYA GPTEVQWHCR ARMVAGANFY IVGRDPAGMP HPETGKDLYE PSHGAKVLTM APGLITLEIV PFRVAAYNKK KKRMDYYDSE HHEDFEFISG TRMRKLAREG QKPPEGFMAP KAWTVLTEYY KSLEKA
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Produktmerkmale
- PAPSS1, 24-624aa, Human, His tag, E.coli
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Aufreinigung
- > 85% by SDS-PAGE
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3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1) (AA 1-624) protein (His tag)
custom-made PAPSS1 Spezies: Human Wirt: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1) (AA 1-624) protein (His tag)custom-made PAPSS1 Spezies: Maus Wirt: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1) (AA 1-603) protein (GST tag)PAPSS1 Spezies: Human Wirt: Wheat germ Recombinant ELISA, WB, AP, AA
3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1) protein (Myc-DYKDDDDK Tag)PAPSS1 Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 0.5 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 0.1M NaCl
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Handhabung
- Avoid repeated freezing and thawing cycles
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- PAPSS1 (3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1))
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Andere Bezeichnung
- PAPSS1
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Hintergrund
- PAPSS1, also known as bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1, is a bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway, yielding 3'-phosphoadenylylsulfate (PAPS). PAPSS1 is also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells. Recombinant human PAPSS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
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Molekulargewicht
- 70.9 kDa (626aa)
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NCBI Accession
- NP_005434
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Pathways
- Glycosaminoglycan Metabolic Process, Ribonucleoside Biosynthetic Process
Target
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