PHOSPHO2 Protein (AA 1-241) (His tag)

Produktnummer ABIN668005

Recombinant PHOSPHO2-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für PHOSPHO2 Protein (AA 1-241) (His tag) (ABIN668005)

Target: PHOSPHO2 (Phosphatase, Orphan 2 (PHOSPHO2))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 85 %

Produktdetails

Proteineigenschaft: AA 1-241

Aufreinigungstag / Konjugat: Dieses PHOSPHO2 Protein ist gelabelt mit His tag.

Sequenz: MGSSHHHHHH SSGLVPRGSH MGSHMKILLV FDFDNTIIDD NSDTWIVQCA PNKKLPIELR DSYRKGFWTE FMGRVFKYLG DKGVREHEMK RAVTSLPFTP GMVELFNFIR KNKDKFDCII ISDSNSVFID WVLEAASFHD IFDKVFTNPA AFNSNGHLTV ENYHTHSCNR CPKNLCKKVV LIEFVDKQLQ QGVNYTQIVY IGDGGNDVCP VTFLKNDDVA MPRKGYTLQK TLSRMSQNLE PMEYSVVVWS SGVDIISHLQ FLIKD

Produktmerkmale: PHOSPHO2, 1-241aa, Human, His tag, E.coli

Aufreinigung: > 85% by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol, 0.1M NaCl

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: PHOSPHO2 (Phosphatase, Orphan 2 (PHOSPHO2))

Andere Bezeichnung: PHOSPHO2

Hintergrund: Pyridoxal phosphate phosphatase PHOSPHO2, orphan 2, also known as PHOSPHO2, belongs to the haloacid dehalogenase (HAD) superfamily. Phosphatase has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). PHOSPHO 1, a phosphoethanolamine/phosphocholine phosphatase, is upregulated in mineralising cells and is thought to be involved in the generation of inorganic phosphate for bone mineralization. PHOSPHO2 is a putative phosphatase sharing 42 % sequence identity with PHOSPHO1. PHOSPHO1 and PHOSPHO2 are very similar, but surprisingly, recombinant PHOSPHO2 hydrolyses phosphoethanolamine and phosphocholine relatively poorly. Recombinant human PHOSPHO2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 30.3 kDa (265aa) confirmed by MALDI-TOF

NCBI Accession: NP_001008489