ABO protein (HIS)

Name: ABO

Hergestellt in: Escherichia Coli (E. coli)

Herkunft (Gen): Human

Konjugat: HIS

Applikation: SDS-PAGE (SDS)

Produktnummer: ABIN667951

Produktbeschreibung

Produktmerkmale: ABO, 54-354aa, Human, His tag, E.coli

Format: Liquid

Beschreibung: ABO, also known as NAGAT, belongs to the glycosyltransferase 6 family. This protein is the basis of the ABO blood group system and related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. Recombinant human ABO protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Histo-blood group ABO system transferase, A3GALNT, A3GALT1, GTB, NAGAT. NCBI no.: NP_065202

Molekulargewicht: 37.4kDa (322aa), confirmed by MALDI-TOF

Anwendungen

Konzentration: 0.5mg/ml (determined by Bradford assay)

Reinheit: > 85% by SDS - PAGE

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200mM NaCl

Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Bilder

ABO protein (HIS)