ADA Protein (AA 1-363) (His tag)

Produktnummer ABIN667926

Dieses Recombinant ADA-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für ADA Protein (AA 1-363) (His tag) (ABIN667926)

Target: ADA (Adenosine Deaminase (ADA))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Reinheit: > 90 %

Produktdetails

Proteineigenschaft: AA 1-363

Aufreinigungstag / Konjugat: Dieses ADA Protein ist gelabelt mit His tag.

Sequenz: MAQTPAFDKP KVELHVHLDG SIKPETILYY GRRRGIALPA NTAEGLLNVI GMDKPLTLPD FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV RYSPHLLANS KVEPIPWNQA EGDLTPDEVV ALVGQGLQEG ERDFGVKARS ILCCMRHQPN WSPKVVELCK KYQQQTVVAI DLAGDETIPG SSLLPGHVQA YQEAVKSGIH RTVHAGEVGS AEVVKEAVDI LKTERLGHGY HTLEDQALYN RLRQENMHFE ICPWSSYLTG AWKPDTEHAV IRLKNDQANY SLNTDDPLIF KSTLDTDYQM TKRDMGFTEE EFKRLNINAA KSSFLPEDEK RELLDLLYKA YGMPPSASAG QNL

Produktmerkmale: ADA, 1-363aa, Human, His tag, E.coli

Aufreinigung: > 90% by SDS-PAGE

Endotoxin-Niveau: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is >40unit/mg, and is defined as the amount of enzyme that convert 1.0 umol of adenosine to inosine per minute at pH 7.5 at 25C.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 1 mM DTT

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: ADA (Adenosine Deaminase (ADA))

Andere Bezeichnung: Adenosine Deaminase/ADA

Hintergrund: ADA, also known as adenosine deaminase, catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. This protein plays an important role in purine metabolism and in adenosine homeostasis. ADA acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Recombinant human ADA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Molekulargewicht: 42.9 kDa (383aa) confirmed by MALDI-TOF

NCBI Accession: NP_000013

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process