AP1S2 Protein (AA 1-157) (His tag)
Kurzübersicht für AP1S2 Protein (AA 1-157) (His tag) (ABIN667919)
Target
Alle AP1S2 Proteine anzeigenProtein-Typ
Spezies
Quelle
Applikation
Reinheit
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Proteineigenschaft
- AA 1-157
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Aufreinigungstag / Konjugat
- Dieses AP1S2 Protein ist gelabelt mit His tag.
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Sequenz
- MQFMLLFSRQ GKLRLQKWYV PLSDKEKKKI TRELVQTVLA RKPKMCSFLE WRDLKIVYKR YASLYFCCAI EDQDNELITL EIIHRYVELL DKYFGSVCEL DIIFNFEKAY FILDEFLLGG EVQETSKKNV LKAIEQADLL QEEAETPRSV LEEIGLT
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Produktmerkmale
- AP1S2, 1-157aa, Human, His tag, E.coli
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Aufreinigung
- > 85% by SDS-PAGE
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Adaptor-Related Protein Complex 1, sigma 2 Subunit (AP1S2) (AA 1-160) protein (His tag)
custom-made AP1S2 Spezies: Maus Wirt: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Adaptor-Related Protein Complex 1, sigma 2 Subunit (AP1S2) (AA 1-157) protein (His tag)custom-made AP1S2 Spezies: Human Wirt: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Adaptor-Related Protein Complex 1, sigma 2 Subunit (AP1S2) (AA 1-157) protein (GST tag)AP1S2 Spezies: Human Wirt: Wheat germ Recombinant ELISA, WB, AA, AP
Adaptor-Related Protein Complex 1, sigma 2 Subunit (AP1S2) (AA 1-157) protein (His-IF2DI Tag)AP1S2 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 0.5 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 40 % glycerol, 0.1M NaCl,2 mM DTT
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Handhabung
- Avoid repeated freezing and thawing cycles
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- AP1S2 (Adaptor-Related Protein Complex 1, sigma 2 Subunit (AP1S2))
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Andere Bezeichnung
- AP1S2
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Hintergrund
- AP1S2, also known as AP-1 complex subunit sigma-2, serves as the small subunit of AP-1 complex 1 and is a member of the adaptin protein family. Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. Recombinant human AP1S2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
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Molekulargewicht
- 20.7 kDa (177aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_003907
Target
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