HSD17B8 Protein (AA 1-261) (His tag)
Kurzübersicht für HSD17B8 Protein (AA 1-261) (His tag) (ABIN667899)
Target
Alle HSD17B8 Proteine anzeigenProtein-Typ
Spezies
Quelle
Applikation
Reinheit
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Proteineigenschaft
- AA 1-261
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Aufreinigungstag / Konjugat
- Dieses HSD17B8 Protein ist gelabelt mit His tag.
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Sequenz
- MASQLQNRLR SALALVTGAG SGIGRAVSVR LAGEGATVAA CDLDRAAAQE TVRLLGGPGS KEGPPRGNHA AFQADVSEAR AARCLLEQVQ ACFSRPPSVV VSCAGITQDE FLLHMSEDDW DKVIAVNLKG TFLVTQAAAQ ALVSNGCRGS IINISSIVGK VGNVGQTNYA ASKAGVIGLT QTAARELGRH GIRCNSVLPG FIATPMTQKV PQKVVDKITE MIPMGHLGDP EDVADVVAFL ASEDSGYITG TSVEVTGGLF M
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Produktmerkmale
- HSD17B8, 1-261aa, Human, His tag, E.coli
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Aufreinigung
- > 95% by SDS-PAGE
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Hydroxysteroid (17-Beta) Dehydrogenase 8 (HSD17B8) (AA 1-259) protein (His tag)
custom-made HSD17B8 Spezies: Maus Wirt: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Hydroxysteroid (17-Beta) Dehydrogenase 8 (HSD17B8) (AA 1-261) protein (His tag)custom-made HSD17B8 Spezies: Human Wirt: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Hydroxysteroid (17-Beta) Dehydrogenase 8 (HSD17B8) (AA 1-261) protein (GST tag)HSD17B8 Spezies: Human Wirt: Wheat germ Recombinant ELISA, WB, AP, AA
Hydroxysteroid (17-Beta) Dehydrogenase 8 (HSD17B8) (AA 1-261) protein (His-IF2DI Tag)HSD17B8 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Hydroxysteroid (17-Beta) Dehydrogenase 8 (HSD17B8) protein (Myc-DYKDDDDK Tag)HSD17B8 Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 0.5 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 40 % glycerol, 150 mM NaCl
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Handhabung
- Avoid repeated freezing and thawing cycles
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- HSD17B8 (Hydroxysteroid (17-Beta) Dehydrogenase 8 (HSD17B8))
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Andere Bezeichnung
- 17 beta-HSD8/HSD17B8
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Hintergrund
- HSD17B8, also known as estradiol 17-beta-dehydrogenase 8, belongs to the short-chain dehydrogenases/reductases (SDR) family. In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. It may play a role in biosynthesis of fatty acids in mitochondria. Recombinant human HSD17B8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
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Molekulargewicht
- 29.1 kDa (281aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_055049
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Pathways
- Steroid Hormone Biosynthesis
Target
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